The toxic effects of 4-hydroperoxycyclophosphamide (4HC) on different human hematopoietic progenitor cells were determined. Day 7 colonies supported by granulocyte colony stimulating factor (G-CSF), day 10 colonies supported by granulocyte-macrophage colony stimulating factor (GM-CSF) were counted. Approximately 2 ⨯ 102 granulocyte-macrophage colonies per 5 ⨯ 104 bone marrow (BM) mononuclear cells were formed in each assy system. A combination of interleukin 3 (IL3) and erythropoietin (EPO) induces 3 types of day 14 colonies ; erythroid burst-forming units (BFU-E, 40±29/5 ⨯ 104 BM cells), granulocytes-macrophage colony forming units (CFU-GM, 143±29/5 ⨯ 104 cells) and mixed colonies (CFU-GEMM, 24±13/5 ⨯ 104 cells). After treatment with 4HC, all the colonies were reduced. However, the recovery rate of day 14 colonies supported by the combination of IL3 and EPO (mean 12.1%) was significantly higher than the recovery rate of both day 7 colonies supported by G-CSF (mean 1.0%) and day 10 colonies supported by GM-CSF (mean 4.0%). The recovery rate of colonies supported by IL3 and EPO was also higher than that of day 14 colonies supported by GM-CSF and EPO. Immature pluripotent hematopoietic progenitor cells supported by IL3 and EPO appeared less sensitive to 4HC treatment than those supported by G-CSF and GM-CSF. Colony forming assays using IL3 may be usefull in order to predict the hematological reconstitution after autologous bone marrow transplantation with 4HC treated graft.
- Interleukin 3
- autologous bone marrow transplantation
- hematopoietic progenitor cells
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)