Presence of protein-bound unconjugated bile acids in the cytoplasmic fraction of rat brain

Nariyasu Mano, Takaaki Goto, Masashi Uchida, Koji Nishimura, Masayuki Ando, Norihiro Kobayashi, Junichi Goto

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Using liquid chromatography/electrospray ionization mass spectrometry, we have found three unconjugated bile acids [cholic acid (CA), chenodeoxycholic acid (CDCA), and deoxycholic acid (DCA)] in the rat brain cytoplasmic fraction. CDCA was detected only upon extraction with high concentrations of guanidine, indicating that it is bound noncovalently to protein in the brain. The most abundant of the three, it was present at a concentration of 1.6 nmol/g wet weight (∼15 mg of protein) of brain, corresponding to almost 30 times its serum concentration. CA and DCA were present at 1/30th the concentration of CDCA. Bile acids conjugated with amino acids, sulfuric acid, and glucuronic acid were not detected. These data clearly demonstrate that unconjugated CDCA and, to a lesser extent, CA and DCA, exists in the rat brain.

Original languageEnglish
Pages (from-to)295-300
Number of pages6
JournalJournal of lipid research
Issue number2
Publication statusPublished - 2004 Feb


  • Chenodeoxycholic acid
  • Cholic acid
  • Deoxycholic acid
  • Liquid chromatography/mass spectrometry

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology


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