Presence of phosphatidylcholine hydroperoxide in human plasma

Teruo Miyazawa, Keiichi Yasuda, Kenshiro Fujimoto, Takashi Kaneda

Research output: Contribution to journalArticlepeer-review

101 Citations (Scopus)

Abstract

A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was newly developed and used for the hydroperoxide-specific determination of phosphatidylcholine hydroperoxide (PCOOH) in human plasma. The method involves separation of phosphatidylcholine derivatives from plasma lipids by normal phase HPLC and subsequent detection of hydroperoxide-dependent chemiluminescence (CL) of PCOOH. CL was produced through luminol oxidation during the reaction of the hydroperoxide and cyto-chrome c-heme. The high specificity for the hydroperoxide allows the sensitive assaying of a large PCOOH range over a concentration range of 50-2,000 pmol of hydroperoxide-O2. Using this method, the occurrence of PCOOH in normal human plasma was strongly suggested and was confirmed quantitatively.

Original languageEnglish
Pages (from-to)744-746
Number of pages3
JournalJournal of biochemistry
Volume103
Issue number5
DOIs
Publication statusPublished - 1988 May

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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