Presence of peroxyradicals in cigarette smoke and the scavenging effect of shikonin, a naphthoquinone pigment

Masahiro Nishizawa, Masahiro Kohno, Minemitsu Nishimura, Akio Kitagawa, Yoshimi Niwano

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Using a new method having been developed for the purpose of quantitative determination for peroxyradicals, the presence of peroxyradicals was proved in cigarette smoke. In brief, peroxyradicals in cigarette smoke were measured by ESR spectrometry coupled to non-reductive scavenging of 1,1-diphenyl-2- picrylhydrazyl (DPPH). As a result, peroxyradicals were found to be major reactive oxygen species (ROS) since the concentration of peroxyradicals recovered from cigarette smoke was much higher than that of any of other ROS (superoxide and hydroxyl radical) and nitric oxide. Furthermore, several antioxidants (ascorbic acid, reduced glutathione, epigallocatechin gallate, shikonin) were examined for scavenging activity against peroxyradicals in the cigarette smoke. Among them shikonin alone exerted the scavenging activity, suggesting that shikonin is promising antioxidant for cigarette filters because of its effectiveness against broad range of ROS including peroxyradicals, heat resistance, nonvolatility and high affinity to the filter.

Original languageEnglish
Pages (from-to)796-799
Number of pages4
JournalChemical and Pharmaceutical Bulletin
Volume53
Issue number7
DOIs
Publication statusPublished - 2005 Jul

Keywords

  • Cigarette smoke
  • ESR
  • Peroxyradical
  • Scavenging activity
  • Shikonin

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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