Presence of immunoreactive endothelin in human milk

Hing Chung Lam, Kazuhiro Takahashi, Mohammed A. Ghatei, Stephen R. Bloom

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Endothelin-like immunoreactivity was detected in human milk at a concentration of 6.8 ± 1.6 pmol l(mean ± SEM; n = 16) using a highly sensitive radioimmunoassay. Gel filtration and fast protein liquid chromatography (FPLC) verified the identity of the endothelin. FPLC revealed 4 peaks, one eluting just after the void volume, and the other three in the positions of endothelin-1, -2, and -3, respectively.

Original languageEnglish
Pages (from-to)184-186
Number of pages3
JournalFEBS Letters
Volume261
Issue number1
DOIs
Publication statusPublished - 1990 Feb 12
Externally publishedYes

Keywords

  • Chromatography
  • Endothelin
  • Human milk
  • Radioimmunoassay

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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