Presence and characterization of multiple mantle lysozymes in the Pacific oyster, Crassostrea gigas

Naoki Itoh, Yuki Okada, Keisuke G. Takahashi, Makoto Osada

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Mantle tissue extracts from the Pacific oyster, Crassostrea gigas, exhibited anti-Gram-positive bacterial and lysozyme activities over a wide pH range, suggesting that multiple defensive mantle lysozymes were present. Degenerated reverse-transcription PCR detected the expression of two mantle lysozymes, CGL-1 and a novel lysozyme CGL-3, confirming the presence of multiple lysozymes in the mantle. Since CGL-3 is a cognate protein of the digestive lysozyme CGL-2, it is assumed that CGL-3 has evolved specifically a defensive function. Functional assays using recombinant CGL-1 and CGL-3 suggested that CGL-1 and CGL-3 play a major defensive role in the mantle tissue, and that they are responsible for lysozyme activity under different pH, ionic strength and temperature conditions. Based on these observations, we conclude that multiple mantle lysozymes in the Pacific oyster are better for host-defense under broader conditions than a single lysozyme.

Original languageEnglish
Pages (from-to)126-135
Number of pages10
JournalFish and Shellfish Immunology
Volume29
Issue number1
DOIs
Publication statusPublished - 2010 Jul

Keywords

  • Adaptive evolution
  • Humoral immunity
  • Lysozyme
  • Mantle
  • Pacific oyster

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

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