Prepartion and antigenic properties of estriol 16-glucuronide- and estradiol 17-glucuronide-[C-6]-bovine serum albumin conjugates

Toshio Nambara, Kazutake Shimada, Tadashi Ohkubo, Toshifumi Niwa

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

The preparation and antigenic properties of estriol 16-glucuronide-bovine serum albumin (BSA) and estradiol 17-glucuronide-BSA conjugates in which the hapten molecule is linked to the carrier protein through an (O-carboxymethyl)oxime bridge at the C-6 position on the steroid nucleus, have been described. Antibodies raised against the two immunogens in the rabbit possessed excellent specificity to estriol 16-glucuronide and estradiol 17-glucuronide, respectively, exhibiting no significant cross-reactions with estrogen glucuronides having a substituent in ring A and no cross-reactivities with free estrogens. their sulfates and related steroids.

Original languageEnglish
Pages (from-to)533-538
Number of pages6
JournalJournal of Steroid Biochemistry
Volume16
Issue number4
DOIs
Publication statusPublished - 1982 Apr

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Fingerprint Dive into the research topics of 'Prepartion and antigenic properties of estriol 16-glucuronide- and estradiol 17-glucuronide-[C-6]-bovine serum albumin conjugates'. Together they form a unique fingerprint.

Cite this