Preparative, electroenzymatic reduction of NAD+ to NADH on a thin poly(acrylic acid) layer-coated graphite felt electrode coimmobilizing ion-paired methyl viologen-cation-exchange polymer and diaphorase

Yoshitomo Kashiwagi, Yoshinori Yanagisawa, Norihiko Shibayama, Kaname Nakahara, Futoshi Kurashima, Jun Ichi Anzai, Tetsuo Osa

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10 Citations (Scopus)

Abstract

A thin poly(acrylic acid) layer-coated graphite felt electrode coimmobilizing ion-paired methyl viologen - cation-exchange polymer and diaphorase was prepared and applied to preparative electroenzymatic reduction of NAD+ in phosphate buffer at constant potential of - 0.80 V vs. SCE. The electrode was stable for the reaction and yielded NADH quantitatively in 97.8% current efficiency.

Original languageEnglish
Pages (from-to)1093-1094
Number of pages2
JournalChemistry Letters
Issue number12
DOIs
Publication statusPublished - 1996 Jan 1

ASJC Scopus subject areas

  • Chemistry(all)

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