Preparations of hammerhead ribozymes for investigations of their cleavable sequences.

Hisaaki Tateoka, Ikumi Kawahara, Satomi Hasegawa, Kaichiro Haruta, Yoshinori Kondo, Chojiro Kojima, Yoshiyuki Tanaka

Research output: Contribution to journalArticlepeer-review


Recently, in hammerhead ribozymes, newly identified loop-loop interaction was found to be important for their activation. Therefore, we chemically synthesized a hammerhead ribozyme with this extra loop sequences and its mutant ribozymes, as well as their substrate RNA strands in order to clarify their cleavable sequences. After purification with an anion exchange column chromatography, we were able to obtain 44mer and 20mer RNA.

Original languageEnglish
Pages (from-to)277-278
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number53
Publication statusPublished - 2009

ASJC Scopus subject areas

  • Medicine(all)


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