Preparations of hammerhead ribozymes for investigations of their cleavable sequences.

Hisaaki Tateoka; Ikumi Kawahara; Satomi Hasegawa; Kaichiro Haruta; Yoshinori Kondo; Chojiro Kojima; Yoshiyuki Tanaka

doi:10.1093/nass/nrp139

Preparations of hammerhead ribozymes for investigations of their cleavable sequences.

Hisaaki Tateoka, Ikumi Kawahara, Satomi Hasegawa, Kaichiro Haruta, Yoshinori Kondo, Chojiro Kojima, Yoshiyuki Tanaka

PHA - Molecular Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

Abstract

Recently, in hammerhead ribozymes, newly identified loop-loop interaction was found to be important for their activation. Therefore, we chemically synthesized a hammerhead ribozyme with this extra loop sequences and its mutant ribozymes, as well as their substrate RNA strands in order to clarify their cleavable sequences. After purification with an anion exchange column chromatography, we were able to obtain 44mer and 20mer RNA.

Original language	English
Pages (from-to)	277-278
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	53
DOIs	https://doi.org/10.1093/nass/nrp139
Publication status	Published - 2009

ASJC Scopus subject areas

Medicine(all)

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abstract = "Recently, in hammerhead ribozymes, newly identified loop-loop interaction was found to be important for their activation. Therefore, we chemically synthesized a hammerhead ribozyme with this extra loop sequences and its mutant ribozymes, as well as their substrate RNA strands in order to clarify their cleavable sequences. After purification with an anion exchange column chromatography, we were able to obtain 44mer and 20mer RNA.",

author = "Hisaaki Tateoka and Ikumi Kawahara and Satomi Hasegawa and Kaichiro Haruta and Yoshinori Kondo and Chojiro Kojima and Yoshiyuki Tanaka",

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AU - Tateoka, Hisaaki

AU - Kawahara, Ikumi

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AU - Kondo, Yoshinori

AU - Kojima, Chojiro

AU - Tanaka, Yoshiyuki

PY - 2009

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AB - Recently, in hammerhead ribozymes, newly identified loop-loop interaction was found to be important for their activation. Therefore, we chemically synthesized a hammerhead ribozyme with this extra loop sequences and its mutant ribozymes, as well as their substrate RNA strands in order to clarify their cleavable sequences. After purification with an anion exchange column chromatography, we were able to obtain 44mer and 20mer RNA.

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Preparations of hammerhead ribozymes for investigations of their cleavable sequences.

Abstract

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