Preparation of specific antiserum to estriol 3-sulfate 16-glucuronide

Nambara Toshio, Niwa Toshifumi, Shimada Kazutake

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5 Citations (Scopus)


The preparation and antigenic properties of estriol 3-sulfate 16-glucuronide-bovine serum albumin (BSA) conjugate in which the hapten is linked to the carrier through an (O-carboxymethyl)oxime bridge at the C-6 position on the steroid nucleus, have been described. Coupling of 6-oxoestriol 3-sulfate 16-glucuronide acetate-methyl ester 6-(O-carboxymethyl)oxime with BSA by the activated ester method followed by removal of the protecting groups with alkali provided the desired conjugate. The antisera raised against the conjugate in rabbits were highly specific to the double conjugate, estriol 3-sulfate 16-glucuronide, discriminating from ring A or D monoconjugated and unconjugated estrogens. The specificity of antisera elicited has been discussed on the basis of stereochemistry of the hapten-[C-6]-BSA conjugate.

Original languageEnglish
Pages (from-to)199-204
Number of pages6
JournalJournal of Steroid Biochemistry
Issue number2
Publication statusPublished - 1984 Aug
Externally publishedYes


  • estriol 16-glucuronide = 3,17β-dihydroxy-1,3,5(10)-estratrien-16α - γl-β-d-glucopyranosiduronate
  • estriol = 1,3,5(10)-estratriene-3,16α, 17β-triol

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology


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