TY - JOUR
T1 - Preparation of specific antiserum to estriol 3-sulfate 16-glucuronide
AU - Toshio, Nambara
AU - Toshifumi, Niwa
AU - Kazutake, Shimada
N1 - Funding Information:
as a titer. The binding affinity was determinedb y incubating a constant amount of antiserumw ith an increasing amount of the non-labelled antigen. The ratio of the bound to free (B/F) observed with Acknowledgements-The authors express their sincere thankst o ProfessorT . Ido, CyclotronR adioisotopeC enter of this University,f or his kind help.T hey are also indebted to all the staff of central analytical laboratory of this Institutef or elementaal nalysesa nd spectraml easurements. This work was supportedi n part by a grant from the Ministry of Education,S ciencea nd Culture of Japan.
PY - 1984/8
Y1 - 1984/8
N2 - The preparation and antigenic properties of estriol 3-sulfate 16-glucuronide-bovine serum albumin (BSA) conjugate in which the hapten is linked to the carrier through an (O-carboxymethyl)oxime bridge at the C-6 position on the steroid nucleus, have been described. Coupling of 6-oxoestriol 3-sulfate 16-glucuronide acetate-methyl ester 6-(O-carboxymethyl)oxime with BSA by the activated ester method followed by removal of the protecting groups with alkali provided the desired conjugate. The antisera raised against the conjugate in rabbits were highly specific to the double conjugate, estriol 3-sulfate 16-glucuronide, discriminating from ring A or D monoconjugated and unconjugated estrogens. The specificity of antisera elicited has been discussed on the basis of stereochemistry of the hapten-[C-6]-BSA conjugate.
AB - The preparation and antigenic properties of estriol 3-sulfate 16-glucuronide-bovine serum albumin (BSA) conjugate in which the hapten is linked to the carrier through an (O-carboxymethyl)oxime bridge at the C-6 position on the steroid nucleus, have been described. Coupling of 6-oxoestriol 3-sulfate 16-glucuronide acetate-methyl ester 6-(O-carboxymethyl)oxime with BSA by the activated ester method followed by removal of the protecting groups with alkali provided the desired conjugate. The antisera raised against the conjugate in rabbits were highly specific to the double conjugate, estriol 3-sulfate 16-glucuronide, discriminating from ring A or D monoconjugated and unconjugated estrogens. The specificity of antisera elicited has been discussed on the basis of stereochemistry of the hapten-[C-6]-BSA conjugate.
KW - estriol 16-glucuronide = 3,17β-dihydroxy-1,3,5(10)-estratrien-16α - γl-β-d-glucopyranosiduronate
KW - estriol = 1,3,5(10)-estratriene-3,16α, 17β-triol
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U2 - 10.1016/0022-4731(84)90384-4
DO - 10.1016/0022-4731(84)90384-4
M3 - Article
C2 - 6482430
AN - SCOPUS:0021261119
SN - 0960-0760
VL - 21
SP - 199
EP - 204
JO - Journal of Steroid Biochemistry
JF - Journal of Steroid Biochemistry
IS - 2
ER -