Preparation of spatially ordered multilayer thin films of antibody and their binding properties

T. Hoshi, H. Saiki, J. I. Anzai

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Spatially ordered multilayer thin films containing anti-fluoresceinisothiocyanate (anti-FITC) were prepared on the surface of a quartz slide to study the binding properties of the multilayer films. A quartz slide was treated in solutions of avidin and biotin-labeled anti-FITC alternately and repeatedly to form multilayer thin films through a strong affinity between avidin and biotin. A spectrophotometric study revealed explicitly that the thin films thus prepared consisted of alternate monomolecular layers of avidin and biotin-labeled anti-FITC. The antibody retained its binding activity to antigen in the multilayer thin film, though the antigen could not access the antibody embedded deep in the multilayer film. Only the outermost four or five layers of antibody were involved in the binding of antigen.

Original languageEnglish
Pages (from-to)623-628
Number of pages6
JournalBiosensors and Bioelectronics
Volume15
Issue number11-12
DOIs
Publication statusPublished - 2000

Keywords

  • Antibody
  • Avidin
  • Biotin
  • Layer-by-layer deposition
  • Multilayer thin film

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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