Preparation of silica-coated quantum dot nanoparticle colloid solutions and their application in in-vivo fluorescence imaging

Yoshio Kobayashi; Hiromu Matsudo; Yohsuke Kubota; Tomohiko Nakagawa; Kohsuke Gonda; Noriaki Ohuchi

doi:10.1252/jcej.14we218

Preparation of silica-coated quantum dot nanoparticle colloid solutions and their application in in-vivo fluorescence imaging

Yoshio Kobayashi, Hiromu Matsudo, Yohsuke Kubota, Tomohiko Nakagawa, Kohsuke Gonda, Noriaki Ohuchi

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

This paper describes three findings. The first is a method for producing colloidal solutions of quantum dot (QD) nanoparticles with silica shells (QD/SiO₂). QD nanoparticles averaging 10.3±2.1 nm in size were coated with silica via a sol–gel reaction with tetraethyl orthosilicate using NaOH as a catalyst. The QD/SiO₂ particle size could be varied by varying the QD concentration. The average particle sizes were 19.1±3.0 (S-QD/SiO₂) and 47.0×6.1 nm (L-QD/SiO₂) for QD concentrations of 6.4×10^-9 M (4.6×10¹¹ particles/L) and 6.4×10^-10 M (4.6-10 particles/L), respectively. The second finding is a method to modify the particle surface with poly(ethylene glycol), which is called PEGylation (QD/SiO₂/PEG). S-QD/SiO₂ and L-QD/SiO₂ were PEGylated using methoxy polyethylene glycol silane (S-QD/SiO₂/PEG and L-QD/SiO₂/PEG, respectively). The third finding is an in-vivo fluorescence imaging technique using the QD/SiO₂/PEG particle colloid solutions. Both QD/ SiO₂/PEG particle colloid solutions fluoresced with intensities comparable with that of the QD colloid solution. Mouse tissues could be imaged by injecting the QD/SiO₂/PEG colloid solution into them and measuring the emitted fluorescence intensity. The L-QD/SiO₂/PEG particles did not form aggregates in blood, which allowed the particles to reach the tissues more efficiently than the S-QD/SiO₂/PEG particles.

Original language	English
Pages (from-to)	112-117
Number of pages	6
Journal	JOURNAL of CHEMICAL ENGINEERING of JAPAN
Volume	48
Issue number	2
DOIs	https://doi.org/10.1252/jcej.14we218
Publication status	Published - 2015 Feb 20

Keywords

Core–shell
Fluorescence imaging
Nanoparticle
Quantum dot
Silica-coating

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

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10.1252/jcej.14we218

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title = "Preparation of silica-coated quantum dot nanoparticle colloid solutions and their application in in-vivo fluorescence imaging",

abstract = "This paper describes three findings. The first is a method for producing colloidal solutions of quantum dot (QD) nanoparticles with silica shells (QD/SiO2). QD nanoparticles averaging 10.3±2.1 nm in size were coated with silica via a sol–gel reaction with tetraethyl orthosilicate using NaOH as a catalyst. The QD/SiO2 particle size could be varied by varying the QD concentration. The average particle sizes were 19.1±3.0 (S-QD/SiO2) and 47.0×6.1 nm (L-QD/SiO2) for QD concentrations of 6.4×10-9 M (4.6×1011 particles/L) and 6.4×10-10 M (4.6-10 particles/L), respectively. The second finding is a method to modify the particle surface with poly(ethylene glycol), which is called PEGylation (QD/SiO2/PEG). S-QD/SiO2 and L-QD/SiO2 were PEGylated using methoxy polyethylene glycol silane (S-QD/SiO2/PEG and L-QD/SiO2/PEG, respectively). The third finding is an in-vivo fluorescence imaging technique using the QD/SiO2/PEG particle colloid solutions. Both QD/ SiO2/PEG particle colloid solutions fluoresced with intensities comparable with that of the QD colloid solution. Mouse tissues could be imaged by injecting the QD/SiO2/PEG colloid solution into them and measuring the emitted fluorescence intensity. The L-QD/SiO2/PEG particles did not form aggregates in blood, which allowed the particles to reach the tissues more efficiently than the S-QD/SiO2/PEG particles.",

keywords = "Core–shell, Fluorescence imaging, Nanoparticle, Quantum dot, Silica-coating",

author = "Yoshio Kobayashi and Hiromu Matsudo and Yohsuke Kubota and Tomohiko Nakagawa and Kohsuke Gonda and Noriaki Ohuchi",

year = "2015",

month = feb,

day = "20",

doi = "10.1252/jcej.14we218",

language = "English",

volume = "48",

pages = "112--117",

journal = "Journal of Chemical Engineering of Japan",

issn = "0021-9592",

publisher = "Society of Chemical Engineers, Japan",

number = "2",

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TY - JOUR

T1 - Preparation of silica-coated quantum dot nanoparticle colloid solutions and their application in in-vivo fluorescence imaging

AU - Kobayashi, Yoshio

AU - Matsudo, Hiromu

AU - Kubota, Yohsuke

AU - Nakagawa, Tomohiko

AU - Gonda, Kohsuke

AU - Ohuchi, Noriaki

PY - 2015/2/20

Y1 - 2015/2/20

N2 - This paper describes three findings. The first is a method for producing colloidal solutions of quantum dot (QD) nanoparticles with silica shells (QD/SiO2). QD nanoparticles averaging 10.3±2.1 nm in size were coated with silica via a sol–gel reaction with tetraethyl orthosilicate using NaOH as a catalyst. The QD/SiO2 particle size could be varied by varying the QD concentration. The average particle sizes were 19.1±3.0 (S-QD/SiO2) and 47.0×6.1 nm (L-QD/SiO2) for QD concentrations of 6.4×10-9 M (4.6×1011 particles/L) and 6.4×10-10 M (4.6-10 particles/L), respectively. The second finding is a method to modify the particle surface with poly(ethylene glycol), which is called PEGylation (QD/SiO2/PEG). S-QD/SiO2 and L-QD/SiO2 were PEGylated using methoxy polyethylene glycol silane (S-QD/SiO2/PEG and L-QD/SiO2/PEG, respectively). The third finding is an in-vivo fluorescence imaging technique using the QD/SiO2/PEG particle colloid solutions. Both QD/ SiO2/PEG particle colloid solutions fluoresced with intensities comparable with that of the QD colloid solution. Mouse tissues could be imaged by injecting the QD/SiO2/PEG colloid solution into them and measuring the emitted fluorescence intensity. The L-QD/SiO2/PEG particles did not form aggregates in blood, which allowed the particles to reach the tissues more efficiently than the S-QD/SiO2/PEG particles.

AB - This paper describes three findings. The first is a method for producing colloidal solutions of quantum dot (QD) nanoparticles with silica shells (QD/SiO2). QD nanoparticles averaging 10.3±2.1 nm in size were coated with silica via a sol–gel reaction with tetraethyl orthosilicate using NaOH as a catalyst. The QD/SiO2 particle size could be varied by varying the QD concentration. The average particle sizes were 19.1±3.0 (S-QD/SiO2) and 47.0×6.1 nm (L-QD/SiO2) for QD concentrations of 6.4×10-9 M (4.6×1011 particles/L) and 6.4×10-10 M (4.6-10 particles/L), respectively. The second finding is a method to modify the particle surface with poly(ethylene glycol), which is called PEGylation (QD/SiO2/PEG). S-QD/SiO2 and L-QD/SiO2 were PEGylated using methoxy polyethylene glycol silane (S-QD/SiO2/PEG and L-QD/SiO2/PEG, respectively). The third finding is an in-vivo fluorescence imaging technique using the QD/SiO2/PEG particle colloid solutions. Both QD/ SiO2/PEG particle colloid solutions fluoresced with intensities comparable with that of the QD colloid solution. Mouse tissues could be imaged by injecting the QD/SiO2/PEG colloid solution into them and measuring the emitted fluorescence intensity. The L-QD/SiO2/PEG particles did not form aggregates in blood, which allowed the particles to reach the tissues more efficiently than the S-QD/SiO2/PEG particles.

KW - Core–shell

KW - Fluorescence imaging

KW - Nanoparticle

KW - Quantum dot

KW - Silica-coating

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DO - 10.1252/jcej.14we218

M3 - Article

AN - SCOPUS:84923120769

SN - 0021-9592

VL - 48

SP - 112

EP - 117

JO - Journal of Chemical Engineering of Japan

JF - Journal of Chemical Engineering of Japan

IS - 2

ER -

Preparation of silica-coated quantum dot nanoparticle colloid solutions and their application in in-vivo fluorescence imaging

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