Preparation of protein transduction domain-fused peptidyl prolyl cis/trans isomerase pin1

Tsubasa Ohashi, Sumito Teshima, Masafumi Hidaka, Takafumi Uchiday

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The phenotypes of mice lacking peptidyl prolyl cis/trans isomerase Pin1 (Pin1-/-) indicated that deficient Pin1 might be related to a variety of diseases. We created TAT-Pin1, a fusion protein of human immunodeficiency virus 1 trans-activator of transcription factor with Pin1. Treatment of HeLa cells with TAT-Pin1 increased the ratio of the S phase. Moreover, TAT-Pin1 restored the proliferating function of Pin1-/- mouse embryonic fibroblasts which cannot restart proliferation after G0 arrest. These results indicate that TAT-Pin1 is useful in studying the functions of Pin1 and can be developed as a macromolecular drug for diseases related to Pin1 loss.

Original languageEnglish
Pages (from-to)2067-2070
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number10
DOIs
Publication statusPublished - 2010

Keywords

  • Cell cycle
  • Peptidyl prolyl cis/trans isomerase Pin1
  • Protein transduction domain human immunodeficiency virus-1 trans-actuvatir if transcription factor (TAT)

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Preparation of protein transduction domain-fused peptidyl prolyl cis/trans isomerase pin1'. Together they form a unique fingerprint.

Cite this