Preparation of photoreactive derivatives of α‐melanotropin by selective modification of the lysine or tryptophan residue

KOJI MURAMOTO, DOUGLAS I. BUCKLEY, J. RAMACHANDRAN

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    4 Citations (Scopus)

    Abstract

    Photoreactive derivatives of α‐melanotropin were prepared by selective modification of the single tryptophan residue in position 9 or the amino group of the lysine residue in position 11 by reaction with 2‐nitro‐4‐azidophenylsulfenyl chloride. [2‐Nitro‐4‐azidophenylsulfenyl‐Trp9]‐α‐melanotropin was found to be five times as potent as α‐melanotropin in stimulating lipolysis in isolated rabbit adipocytes. [Nε‐2‐nitro‐4‐azidophenylsulfenyl‐Lys11]‐α‐melanotropin had only 11% of the lipolytic potency of α‐melanotropin.

    Original languageEnglish
    Pages (from-to)366-370
    Number of pages5
    JournalInternational Journal of Peptide and Protein Research
    Volume20
    Issue number4
    DOIs
    Publication statusPublished - 1982 Oct

    Keywords

    • 2‐nitro‐4‐azidophenylsulfenyl
    • lipolysis
    • partition chromatography
    • sulfenamide cleavage.

    ASJC Scopus subject areas

    • Biochemistry

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