Preparation of Nafion/polycation layer-by-layer films for adsorption and release of insulin

Kentaro Yoshida, Katsuhiko Sato, Tetsuya Ono, Takenori Dairaku, Yoshitomo Kashiwagi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Thin films were prepared using layer-by-layer (LbL) deposition of Nafion (NAF) and polycations such as poly(allylamine hydrochloride) (PAH), poly(ethyleneimine) (PEI), and poly(diallydimethylammonium chloride) (PDDA). Insulin was then adsorbed on the NAF-polycation LbL films by immersion in an insulin solution. The NAF-polycation LbL films were characterized using a quartz crystal microbalance and an atomic force microscope. The release of insulin from the LbL films was characterized using UV-visible adsorption spectroscopy and fluorescence emission spectroscopy. The greatest amount of insulin was adsorbed on the NAF-PAH LbL film. The amount of insulin adsorbed on the (NAF/PAH)5NAF LbL films by immersion in a 1 mg mL-1 insulin solution at pH 7.4 was 61.8 μg cm-2. The amount of insulin released from the LbL films was higher when immersed in insulin solutions at pH 2.0 and pH 9.0 than at pH 7.4. Therefore, NAF-polycations could be employed as insulin delivery LbL films under mild conditions and as an insulin release control system according to pH change.

Original languageEnglish
Article number812
JournalPolymers
Volume10
Issue number8
DOIs
Publication statusPublished - 2018 Jul 24

Keywords

  • Controlled release
  • Drug delivery
  • Insulin
  • Layer-by-layer film
  • Nafion

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

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