Preparation of multilayer films consisting of glucose oxidase and poly(amidoamine) dendrimer and their stability

K. Suwa, Katsuhiko Sato, J. Anzai

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Multilayer films comprising of glucose oxidase (GOx) and phenylboronic acid-modified poly(amidoamine) dendrimer (PBA-PAMAM) were prepared and their pH- and glucose-induced decomposition was studied. Multilayered GOx/PBA-PAMAM films were prepared through boronate ester linkages between PBA-PAMAM and carbohydrate chains of GOx. In contrast, preparation of multilayer film was unsuccessful when unmodified PAMAM was used in lieu of PBA-PAMAM, confirming an essential role of PBA residues in constructing multilayer films. The ten-layered (GOx/PBA-PAMAM)10 film was stable in aqueous solutions of pH 8.5, whereas the multilayer film decomposed in neutral and acidic media as a result of cleavage of the boronate ester bond. The pH threshold for the film decomposition was pH 7.0–8.0. In addition, the (GOx/PBA-PAMAM)10 film decomposed in glucose solutions owing to the oxidative scission of carbon–boron bond of PBA-PAMAM by enzymatically produced hydrogen peroxide. Thus, the potential use of the (GOx/PBA-PAMAM)10 film for the development of pH- and glucose-sensitive devices is suggested.

Original languageEnglish
Pages (from-to)2713-2718
Number of pages6
JournalColloid and Polymer Science
Volume293
Issue number9
DOIs
Publication statusPublished - 2015 Sep 13

Keywords

  • Dendrimer
  • Glucose oxidase
  • Multilayer film
  • Phenylboronic acid

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Polymers and Plastics
  • Colloid and Surface Chemistry
  • Materials Chemistry

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