Preparation of multilayer films composed of poly(ethyleneimine) and uricase and thier catalytic activities

Hidekazu Saiki; Tomonori Hoshi; Junichi Anzai

doi:10.2116/bunsekikagaku.51.1179

Preparation of multilayer films composed of poly(ethyleneimine) and uricase and thier catalytic activities

Hidekazu Saiki, Tomonori Hoshi, Junichi Anzai

Clinical Research, Innovation and Education Center

Research output: Contribution to journal › Article › peer-review

Abstract

Multilayer films composed of poly(ethyleneime) (PEI) and uricase were prepared by an alternate deposition of PEI and uricase on the surface of a platinum (Pt) electrode for developing amperometric uric acid sensors. PEI and uricase were successfully built into a multilayer film, and the loading of uricase in the film was evaluated using a quartz-crystal microbalance. The Pt electrode coated with the PEI/uricase multilayer film functioned as an amperometric biosensor senstive to uric acid. The output current of the sensor depended linearly on the number of PEI/uricase layers in the film.

Original language	English
Pages (from-to)	1179-1182
Number of pages	4
Journal	BUNSEKI KAGAKU
Volume	51
Issue number	12
DOIs	https://doi.org/10.2116/bunsekikagaku.51.1179
Publication status	Published - 2002 Dec

Keywords

Amperometric sensor
Multilayer film
Poly(ethyleneimine)
Uricase

ASJC Scopus subject areas

Analytical Chemistry

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10.2116/bunsekikagaku.51.1179

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abstract = "Multilayer films composed of poly(ethyleneime) (PEI) and uricase were prepared by an alternate deposition of PEI and uricase on the surface of a platinum (Pt) electrode for developing amperometric uric acid sensors. PEI and uricase were successfully built into a multilayer film, and the loading of uricase in the film was evaluated using a quartz-crystal microbalance. The Pt electrode coated with the PEI/uricase multilayer film functioned as an amperometric biosensor senstive to uric acid. The output current of the sensor depended linearly on the number of PEI/uricase layers in the film.",

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AU - Saiki, Hidekazu

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AU - Anzai, Junichi

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AB - Multilayer films composed of poly(ethyleneime) (PEI) and uricase were prepared by an alternate deposition of PEI and uricase on the surface of a platinum (Pt) electrode for developing amperometric uric acid sensors. PEI and uricase were successfully built into a multilayer film, and the loading of uricase in the film was evaluated using a quartz-crystal microbalance. The Pt electrode coated with the PEI/uricase multilayer film functioned as an amperometric biosensor senstive to uric acid. The output current of the sensor depended linearly on the number of PEI/uricase layers in the film.

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KW - Uricase

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Preparation of multilayer films composed of poly(ethyleneimine) and uricase and thier catalytic activities

Abstract

Keywords

ASJC Scopus subject areas

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