Preparation of multilayer films composed of poly(ethyleneimine) and uricase and thier catalytic activities

Hidekazu Saiki, Tomonori Hoshi, Jun Ichi Anzai

Research output: Contribution to journalArticlepeer-review

Abstract

Multilayer films composed of poly(ethyleneime) (PEI) and uricase were prepared by an alternate deposition of PEI and uricase on the surface of a platinum (Pt) electrode for developing amperometric uric acid sensors. PEI and uricase were successfully built into a multilayer film, and the loading of uricase in the film was evaluated using a quartz-crystal microbalance. The Pt electrode coated with the PEI/uricase multilayer film functioned as an amperometric biosensor senstive to uric acid. The output current of the sensor depended linearly on the number of PEI/uricase layers in the film.

Original languageEnglish
Pages (from-to)1179-1182
Number of pages4
JournalBUNSEKI KAGAKU
Volume51
Issue number12
DOIs
Publication statusPublished - 2002 Dec

Keywords

  • Amperometric sensor
  • Multilayer film
  • Poly(ethyleneimine)
  • Uricase

ASJC Scopus subject areas

  • Analytical Chemistry

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