Abstract
Multilayer films composed of poly(ethyleneime) (PEI) and uricase were prepared by an alternate deposition of PEI and uricase on the surface of a platinum (Pt) electrode for developing amperometric uric acid sensors. PEI and uricase were successfully built into a multilayer film, and the loading of uricase in the film was evaluated using a quartz-crystal microbalance. The Pt electrode coated with the PEI/uricase multilayer film functioned as an amperometric biosensor senstive to uric acid. The output current of the sensor depended linearly on the number of PEI/uricase layers in the film.
Original language | English |
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Pages (from-to) | 1179-1182 |
Number of pages | 4 |
Journal | BUNSEKI KAGAKU |
Volume | 51 |
Issue number | 12 |
DOIs | |
Publication status | Published - 2002 Dec |
Keywords
- Amperometric sensor
- Multilayer film
- Poly(ethyleneimine)
- Uricase
ASJC Scopus subject areas
- Analytical Chemistry