Preparation of layer-by-layer thin films containing insulin and its pH-sensitive decomposition

Kentaro Yoshida, Hiroshi Sato, Shigehiro Takahashi, Jun Ichi Anzai

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


The preparation of layered thin films by taking advantage of the electrostatic force of attraction, hydrogen bonding, and biological affinity between synthetic polymers with the use of a layer-by-layer (LbL) deposition technique was investigated. Insulin was purchased from Wako Pure Chemicals Industries Ltd. A quartz crystal microbalance (QCM) was employed for the gravimetric analysis of LbL films. A 8-MHz AT-cut quartz resonator coated with a thin Au layer was used as a probe. The quartz was mounted in a flow cell and the surface was exposed to 1 mg/mL PEI solution in water for 15 min to deposit a first PEI layer. The PEI-modified quartz resonator was exposed to 0.5 mg/mL polyanion in 10 mM citrate buffer containing 150 mM NaCl for 15 min and rinsed with the working buffer for 5 min. The stability of PVS-insulin LbL film at pH 1.0 was by monitoring frequency changes in QCM evaluated. It was observed that the insulin thin films can be prepared by an alternate deposition of polyanion and insulin at pH 1.0-3.0.

Original languageEnglish
Pages (from-to)90-91
Number of pages2
JournalPolymer Journal
Issue number2
Publication statusPublished - 2008


  • Controlled release
  • Insulin
  • Layer-by-layer
  • Thin film

ASJC Scopus subject areas

  • Polymers and Plastics
  • Materials Chemistry


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