Preparation of insulin-containing microcapsules by a layer-by-layer deposition of concanavalin a and glycogen

Katsuhiko Sato, Daisuke Kodama, Yoshihiro Endo, Jun Ichi Anzai

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

The sugar sensitive microcapsules were prepared by a layer-by-layer deposition of concanavalin A (Con A) and glycogen on a calcium carbonate particle containing fluorescein-labeled insulin (F-insulin). The Con Alglycogen multilayer capsules were formed through sugar-lectin interactions by using inner and outer poly(ethyleneimine)/poly(vinyl sulfate) multilayers as supports, while without the supports the microcapsules could not be formed. Fluorescent microscope observations revealed that the capsules thus prepared are spherical in shape with 3-10 m diameter. The microcapsules released encapsulated F-insulin upon addition of sugars. This is because the added sugars replace glycogen in the binding site of Con A, resulting in the enhanced permeability of the microcapsules to insulin.

Original languageEnglish
Pages (from-to)386-390
Number of pages5
JournalJournal of Nanoscience and Nanotechnology
Volume9
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1

Keywords

  • Concanavalin a
  • Glycogen
  • Insulin
  • Multilayer microcapsule
  • Sugar-sensitive microcapsule

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics

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