Preparation of immunosensors using a microfluidic device with an interdigitated array electrode modified with antibodies

Yu Hirano, Tomoyuki Yasukawa, Yoshihiro Sawayashiki, Hitoshi Shiku, Fumio Mizutani, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We prepared a microfluidic device with an interdigitated array electrode modified with antibodies to develop immunosensors. A comb-type array (W1) was used for forming immunocomplexes and another (W2) for detecting mediators generated by the enzyme reaction. We used electropolymerization and avidin-biotin complexes for an addressable immobilization of antibodies on W1. Since the microfluidics significantly accelerated the formation of the immunocomplexes, a period as short as 10 min was sufficient to detect the responses. The current observed at bare array (W2) increased with increasing analyte (mouse IgG) concentration in the range of 1.0-100 ng/mL. Therefore, present procedure is suitable for rapid immunosensing in a simple device.

Original languageEnglish
Pages (from-to)175-177
Number of pages3
JournalElectrochemistry
Volume78
Issue number2
DOIs
Publication statusPublished - 2010 Feb

Keywords

  • Addressable immobilization
  • Immuno-sensing
  • Microfluidic device

ASJC Scopus subject areas

  • Electrochemistry

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