Preparation of heat-induced artificial collagen gels based on collagen-mimetic dendrimers

Chie Kojima, Tomoyuki Suehiro, Tomoko Tada, Yasuhiro Sakamoto, Tomonori Waku, Naoki Tanaka

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


Collagen, a major component of the extracellular matrix, contains Gly-Pro-Hyp repeats that form a hydrogel. In this study, artificial collagen-mimetic materials were designed. Synthetic dendritic macromolecules were fully modified with (Pro-Hyp-Gly) n and named collagen-mimetic dendrimers. A collagen-like triple helical structure was observed by circular dichroism spectrometry, with an efficiency that depended on the peptide length. A (Pro-Hyp-Gly) 10-modified dendrimer exhibited the most efficient triple helix formation. Thermal stability was enhanced by clustering at the surface of the dendrimer. The (Pro-Hyp-Gly) 10-modified dendrimer was assembled by heating and the assembly was affected by temperature, time and concentration. Hydrogels based on the (Pro-Hyp-Gly) 10-modified dendrimer, but not on the peptide itself, were successfully prepared by heating. The sol-gel transition behavior was similar to natural collagen but not gelatin, which is thermally denatured collagen. Dynamic rheological analysis showed that the sol-gel transition temperature and the strength depended on the concentration. Thus, the collagen-mimetic dendrimer incorporating (Pro-Hyp-Gly) 10 is an injectable and controllable artificial collagen gel.

Original languageEnglish
Pages (from-to)8991-8997
Number of pages7
JournalSoft Matter
Issue number19
Publication statusPublished - 2011 Oct 7
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics


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