Preparation of enzyme multilayers on electrode surface by use of avidin and biotin-labeled enzyme for biosensor applications

Pin Gang He; Takeo Takahashi; Tomonori Hoshi; Junichi Anzai; Yasuo Suzuki; Tetsuo Osa

doi:10.1016/0928-4931(94)90037-X

Preparation of enzyme multilayers on electrode surface by use of avidin and biotin-labeled enzyme for biosensor applications

Pin Gang He, Takeo Takahashi, Tomonori Hoshi, Junichi Anzai, Yasuo Suzuki, Tetsuo Osa

Clinical Research, Innovation and Education Center

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Multilayer membranes composed of avidin and biotin-labeled glucose oxidase (B-GOD) or of an avidin-B-GOD complex and B-GOD were prepared on an indium tin oxide (ITO) electrode, based on the high affinity between avidin and B-GOD. The B-GOD-modified ITO electrodes can be used to determine glucose electrochemically. The magnitude of the output signal (i.e., electric current) of the electrodes depended clearly on the number of enzyme layers.

Original language	English
Pages (from-to)	103-106
Number of pages	4
Journal	Materials Science and Engineering C
Volume	2
Issue number	1-2
DOIs	https://doi.org/10.1016/0928-4931(94)90037-X
Publication status	Published - 1994 Dec

Keywords

Avidin
Biosensors
Biotin
Electrodes
Enzymes

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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10.1016/0928-4931(94)90037-X

Cite this

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title = "Preparation of enzyme multilayers on electrode surface by use of avidin and biotin-labeled enzyme for biosensor applications",

abstract = "Multilayer membranes composed of avidin and biotin-labeled glucose oxidase (B-GOD) or of an avidin-B-GOD complex and B-GOD were prepared on an indium tin oxide (ITO) electrode, based on the high affinity between avidin and B-GOD. The B-GOD-modified ITO electrodes can be used to determine glucose electrochemically. The magnitude of the output signal (i.e., electric current) of the electrodes depended clearly on the number of enzyme layers.",

keywords = "Avidin, Biosensors, Biotin, Electrodes, Enzymes",

author = "He, {Pin Gang} and Takeo Takahashi and Tomonori Hoshi and Junichi Anzai and Yasuo Suzuki and Tetsuo Osa",

note = "Funding Information: This work was supportedin part by Grant-in-Aid for ScientificR esearcohn PriorityA reas( NO.05235102) from Ministry of Education,S ciencea nd Culture of Japan.",

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doi = "10.1016/0928-4931(94)90037-X",

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T1 - Preparation of enzyme multilayers on electrode surface by use of avidin and biotin-labeled enzyme for biosensor applications

AU - He, Pin Gang

AU - Takahashi, Takeo

AU - Hoshi, Tomonori

AU - Anzai, Junichi

AU - Suzuki, Yasuo

AU - Osa, Tetsuo

N1 - Funding Information: This work was supportedin part by Grant-in-Aid for ScientificR esearcohn PriorityA reas( NO.05235102) from Ministry of Education,S ciencea nd Culture of Japan.

PY - 1994/12

Y1 - 1994/12

N2 - Multilayer membranes composed of avidin and biotin-labeled glucose oxidase (B-GOD) or of an avidin-B-GOD complex and B-GOD were prepared on an indium tin oxide (ITO) electrode, based on the high affinity between avidin and B-GOD. The B-GOD-modified ITO electrodes can be used to determine glucose electrochemically. The magnitude of the output signal (i.e., electric current) of the electrodes depended clearly on the number of enzyme layers.

AB - Multilayer membranes composed of avidin and biotin-labeled glucose oxidase (B-GOD) or of an avidin-B-GOD complex and B-GOD were prepared on an indium tin oxide (ITO) electrode, based on the high affinity between avidin and B-GOD. The B-GOD-modified ITO electrodes can be used to determine glucose electrochemically. The magnitude of the output signal (i.e., electric current) of the electrodes depended clearly on the number of enzyme layers.

KW - Avidin

KW - Biosensors

KW - Biotin

KW - Electrodes

KW - Enzymes

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Preparation of enzyme multilayers on electrode surface by use of avidin and biotin-labeled enzyme for biosensor applications

Abstract

Keywords

ASJC Scopus subject areas

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