Preparation of enzyme multilayers on electrode surface by use of avidin and biotin-labeled enzyme for biosensor applications

Pin Gang He, Takeo Takahashi, Tomonori Hoshi, Junichi Anzai, Yasuo Suzuki, Tetsuo Osa

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Multilayer membranes composed of avidin and biotin-labeled glucose oxidase (B-GOD) or of an avidin-B-GOD complex and B-GOD were prepared on an indium tin oxide (ITO) electrode, based on the high affinity between avidin and B-GOD. The B-GOD-modified ITO electrodes can be used to determine glucose electrochemically. The magnitude of the output signal (i.e., electric current) of the electrodes depended clearly on the number of enzyme layers.

Original languageEnglish
Pages (from-to)103-106
Number of pages4
JournalMaterials Science and Engineering C
Volume2
Issue number1-2
DOIs
Publication statusPublished - 1994 Jan 1

Keywords

  • Avidin
  • Biosensors
  • Biotin
  • Electrodes
  • Enzymes

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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