Preparation of cationized polysaccharides as gene transfection carrier for bone marrow-derived mesenchymal stem cells

Jun Ichiro Jo, Arimichi Okazaki, Kentaro Nagane, Masaya Yamamoto, Yasuhiko Tabata

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

The objective of this study is to prepare a non-viral carrier of gene transfection from various polysaccharides and evaluate the feasibility in gene expression for mesenchymal stem cells (MSCs). Various amounts of spermine were chemically introduced into pullulan, dextran and mannan with a molecular weight of around 40 000 or pullulan with different molecular weights to prepare cationized polysaccharides with different extents of spermine introduced (spermine-polysaccharide). Each cationized polysaccharide was complexed with a plasmid DNA at various ratios and in vitro gene transfection was investigated for rat bone marrow-derived MSCs. The level of gene expression depended on the type of cationized polysaccharide. The highest level was observed for the complex of spermine-pullulan and plasmid DNA. Additionally, the level also depended on the molecular weight of pullulan and the extent of spermine introduced to pullulan. Suppression of gene expression with chlorpromazine and methyl-β-cyclodextrin of endocytosis inhibitors demonstrated that the cellular uptake of spermine-pullulan-plasmid DNA complexes was mediated by clathrin- and raft/caveolae-dependent endocytic pathways. The cationized pullulan is a promising non-viral carrier of plasmid DNA for MSCs.

Original languageEnglish
Pages (from-to)185-204
Number of pages20
JournalJournal of Biomaterials Science, Polymer Edition
Volume21
Issue number2
DOIs
Publication statusPublished - 2010 Jan 1
Externally publishedYes

Keywords

  • Asialoglycoprotein receptor
  • Cationized extent
  • Gene expression
  • Mesenchymal stem cells
  • Polysaccharide
  • Spermine

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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