Preparation of avidin-containing polyelectrolyte microcapsules and their uptake and release properties

Yoshihiro Endo, Katsuhiko Sato, Jun Ichi Anzai

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Avidin-containing polyelectrolyte microcapsules were prepared by a layer-by-layer deposition technique and uptake and release of biotin-labelled fluorescein (b-FITC) was studied. The polyelectrolyte microcapsules were prepared by coating the surface of calcium carbonate (CaCO3) microparticles containing avidin-poly(styrene sulfonate) (PSS) conjugate, followed by dissolution of CaCO3 core in an ethylenediaminetetraacetic acid solution. Release of avidin from the microcapsules was markedly suppressed due to formation of a high molecular weight of avidin-PSS conjugate in the microcapsules. The uptake of b-FITC into the microcapsules was highly enhanced through a strong binding of b-FITC to avidin, as compared to the uptake into avidin-free microcapsules. Release of b-FITC from the microcapsules was accelerated upon addition of biotin, 2-iminobiotin, or lipoic acid in the solution due to the competitive binding of the additives to the binding site of avidin.

Original languageEnglish
Pages (from-to)711-720
Number of pages10
JournalPolymer Bulletin
Volume66
Issue number5
DOIs
Publication statusPublished - 2011 Mar

Keywords

  • Avidin
  • Biotin
  • Fluorescein
  • Polyelectrolyte microcapsule
  • Uptake and release

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics
  • Polymers and Plastics
  • Materials Chemistry

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