Abstract
Avidin-containing polyelectrolyte microcapsules were prepared by a layer-by-layer deposition technique and uptake and release of biotin-labelled fluorescein (b-FITC) was studied. The polyelectrolyte microcapsules were prepared by coating the surface of calcium carbonate (CaCO3) microparticles containing avidin-poly(styrene sulfonate) (PSS) conjugate, followed by dissolution of CaCO3 core in an ethylenediaminetetraacetic acid solution. Release of avidin from the microcapsules was markedly suppressed due to formation of a high molecular weight of avidin-PSS conjugate in the microcapsules. The uptake of b-FITC into the microcapsules was highly enhanced through a strong binding of b-FITC to avidin, as compared to the uptake into avidin-free microcapsules. Release of b-FITC from the microcapsules was accelerated upon addition of biotin, 2-iminobiotin, or lipoic acid in the solution due to the competitive binding of the additives to the binding site of avidin.
Original language | English |
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Pages (from-to) | 711-720 |
Number of pages | 10 |
Journal | Polymer Bulletin |
Volume | 66 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2011 Mar |
Keywords
- Avidin
- Biotin
- Fluorescein
- Polyelectrolyte microcapsule
- Uptake and release
ASJC Scopus subject areas
- Chemistry(all)
- Condensed Matter Physics
- Polymers and Plastics
- Materials Chemistry