Preparation of an organized film composed of polymers, avidin, and concanavalin A and its binding properties

Junichi Anzai, Sumiko Akase

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The multilayer thin films composed of polymers, avidin, and concanavalin A (Con A) were prepared on the surface of a quartz slide by alternate deposition of anionic and cationic polymers and the proteins. The loading of avidin and Con A in the film was evaluated spectroscopically by using Texas Red tagged avidin and Con A. Avidin molecules assembled in the film caused its binding activity toward biotin and analogue 2-(4′-hydroxyphenylazo)benzoic acid to be retained. The polymer layers contributed to improving stability of the protein film.

Original languageEnglish
Pages (from-to)361-364
Number of pages4
JournalMacromolecular Bioscience
Volume2
Issue number8
DOIs
Publication statusPublished - 2002 Dec 1

Keywords

  • Avidin
  • Con A
  • Polyamines
  • Polyelectrolytes
  • Thin films

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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