TY - JOUR
T1 - Preparation of an antigen-responsive fluorogenic immunosensor by tyrosine chemical modification of the antibody complementarity determining region
AU - Sato, Shinichi
AU - Matsumura, Masaki
AU - Ueda, Hiroshi
AU - Nakamura, Hiroyuki
N1 - Funding Information:
This work was partially supported by a research grant from a Grant-in-Aid for Scientific Research (B) (19H02848 to S. Sato), JST FOREST Program (Grant Number JPMJFR2005 to S. Sato, Japan JST), and a Grant-in-Aid for Chemistry for Multimolecular Crowding Biosystems (20H04699 to H. Nakamura) from MEXT, Japan. S.S. thanks Chizu Fujimura (Tohoku University) for her help with the revision stage experiments. This work was supported by Tokyo Tech World Research Hub Initiative (WRHI) Program of Institute of Innovative Research, Tokyo Institute of Technology.
Publisher Copyright:
© 2021 The Royal Society of Chemistry.
PY - 2021/9/28
Y1 - 2021/9/28
N2 - Full-length pharmaceutical antibodies, trastuzumab and rituximab, were chemically modified into Quenchbody, a fluorescent immunosensor, using a two-step reaction: (1) selective tyrosine residue modification of antibody complementarity determining regions (CDRs), and (2) introduction of fluorescent dye molecules by Cu-free click reaction. Without the need for genetic manipulation and time-consuming examination of protein expression conditions, the antibody-dye combination with good antigen response efficiency was examined in a simple two-hour operation.
AB - Full-length pharmaceutical antibodies, trastuzumab and rituximab, were chemically modified into Quenchbody, a fluorescent immunosensor, using a two-step reaction: (1) selective tyrosine residue modification of antibody complementarity determining regions (CDRs), and (2) introduction of fluorescent dye molecules by Cu-free click reaction. Without the need for genetic manipulation and time-consuming examination of protein expression conditions, the antibody-dye combination with good antigen response efficiency was examined in a simple two-hour operation.
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U2 - 10.1039/d1cc03231c
DO - 10.1039/d1cc03231c
M3 - Article
C2 - 34477721
AN - SCOPUS:85115949092
VL - 57
SP - 9760
EP - 9763
JO - Chemical Communications
JF - Chemical Communications
SN - 1359-7345
IS - 76
ER -