Preparation of a specifically tritiated locust adipokinetic hormone analog with full biological potency

Koji Muramoto, J. Ramachandran, Pnina Moshitzky, S. W. Applebaum

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    A synthetic peptide related to locus adipokinetic hormone (AKH) and shrimp red pigment concentrating hormone (RPCH) containing a tyrosine residue in place of phenylalanine was iodinated and the 3,5‐diiodotyrosyl derivative was isolated by reverse phase HPLC. Catalytic dehalogenation of the diiodo derivative in the presence of tritium yielded the tritiated AKH analog which was isolated by gel filtration on Sephadex LH‐20 and reverse phase HPLC. The tritiated peptide was formed to be identical to AKH in its ability to stimulate lipid release into the hemolymph of locusts in vivo where the diiodotryrosyl derivative was inactive. The specific radioactivity of the tritiated peptide was 57.2 Ci/mmol, or 99% of the theoretical value.

    Original languageEnglish
    Pages (from-to)443-446
    Number of pages4
    JournalInternational Journal of Peptide and Protein Research
    Volume23
    Issue number4
    DOIs
    Publication statusPublished - 1984 Apr

    Keywords

    • 3,5‐diiodotyrosyl analog of AKH and RPCH
    • adipokinetic hormone
    • locust
    • shrimp red pigment concentrating hormone
    • tritiated analog of AKH and RPCH

    ASJC Scopus subject areas

    • Biochemistry

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