TY - JOUR
T1 - Preparation of a specifically tritiated locust adipokinetic hormone analog with full biological potency
AU - Muramoto, Koji
AU - Ramachandran, J.
AU - Moshitzky, Pnina
AU - Applebaum, S. W.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1984/4
Y1 - 1984/4
N2 - A synthetic peptide related to locus adipokinetic hormone (AKH) and shrimp red pigment concentrating hormone (RPCH) containing a tyrosine residue in place of phenylalanine was iodinated and the 3,5‐diiodotyrosyl derivative was isolated by reverse phase HPLC. Catalytic dehalogenation of the diiodo derivative in the presence of tritium yielded the tritiated AKH analog which was isolated by gel filtration on Sephadex LH‐20 and reverse phase HPLC. The tritiated peptide was formed to be identical to AKH in its ability to stimulate lipid release into the hemolymph of locusts in vivo where the diiodotryrosyl derivative was inactive. The specific radioactivity of the tritiated peptide was 57.2 Ci/mmol, or 99% of the theoretical value.
AB - A synthetic peptide related to locus adipokinetic hormone (AKH) and shrimp red pigment concentrating hormone (RPCH) containing a tyrosine residue in place of phenylalanine was iodinated and the 3,5‐diiodotyrosyl derivative was isolated by reverse phase HPLC. Catalytic dehalogenation of the diiodo derivative in the presence of tritium yielded the tritiated AKH analog which was isolated by gel filtration on Sephadex LH‐20 and reverse phase HPLC. The tritiated peptide was formed to be identical to AKH in its ability to stimulate lipid release into the hemolymph of locusts in vivo where the diiodotryrosyl derivative was inactive. The specific radioactivity of the tritiated peptide was 57.2 Ci/mmol, or 99% of the theoretical value.
KW - 3,5‐diiodotyrosyl analog of AKH and RPCH
KW - adipokinetic hormone
KW - locust
KW - shrimp red pigment concentrating hormone
KW - tritiated analog of AKH and RPCH
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U2 - 10.1111/j.1399-3011.1984.tb02743.x
DO - 10.1111/j.1399-3011.1984.tb02743.x
M3 - Article
C2 - 6724806
AN - SCOPUS:0021243664
VL - 23
SP - 443
EP - 446
JO - International journal of protein research
JF - International journal of protein research
SN - 0367-8377
IS - 4
ER -