Preparation and function of self-organaized lipid nanotubular micro network

Shin Ichiro M. Nomura, Kazunari Akiyoshi

Research output: Contribution to conferencePaperpeer-review

Abstract

Lipid nanotube consisting of lipid bilayer membrane has a high aspect ratio with diameter of several hundreds nanomerer. Recently we reported that the spontaneous formation of the three-dimensional lipid tubular network in the presence of various gangliosides or cholesterol. However, the network was physically fragile, and easy to dismantle into liposomes. This report describes stable lipid nanotube network formation in agarose gel and a large DNA encapsulation in the tube. Agarose solution of low-melting temperature was adopted for forming the lipid nanotube network. The agarose gel stably retained the micro network in the range of concentration from 0.5 to 1.0wt%, with lipid concentration of 1mM. The motion of single DNA molecule (T4DNA, 167kbp) entrapped in the hollow lipid tube was directly observed by fluorescence microscopy in real-time. The DNA shows unidirectional brownian motion along with the lipid nanotube. This result supports that the inner spaces of the tube allows holding large polymer molecules and limiting their movement. The stable lipid nanotube network gel connected with hydrophilic tunnels can be used for constructing a biochemical micro-reactor.

Original languageEnglish
Pages4803-4804
Number of pages2
Publication statusPublished - 2005 Dec 1
Externally publishedYes
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

Keywords

  • Cholesterol
  • Giant liposome
  • Micro reactor
  • Nanotube network
  • Self-organization

ASJC Scopus subject areas

  • Engineering(all)

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