Preparation and characterization of SNO-PEG-hemoglobin as a candidate for oxygen transporting material

K. Nakai, H. Togashi, T. Yasukohchi, I. Sakuma, S. Fujii, M. Yoshioka, H. Satoh, A. Kitabatake

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Acellular hemoglobin (Hb) derivates developed as oxygen carriers are known to cause hypertensive reactions due to their nitric oxide (NO) scavenging action. To modulate this undesired activity, we have developed a new Hb derivative, s-nitrosylated polyethylene glycol (PEG)-modified hemoglobin (SNO-PEG-Hb), which can deliver oxygen and NO. After human Hb was modified with PEG to increase its molecular weight, the free sulfhydryl groups of Hb were s-nitrosylated with s-nitrosoglutathione. Administration of unmodified Hb into anesthetized rats caused a hypertensive reaction, while s-nitrosylated Hb derivatives such as SNO-Hb and SNO-PEG-Hb did not raise blood pressure. The plasma half-lives of heme and NO bound to SNO-PEG-Hb were 11.5 and 2.4 hours respectively, indicating that the s-nitrosylated Hb derivative may act as a slow-releasing agent for NO. Based on these findings, SNO-PEG-Hb is a useful candidate for a blood substitute and tool for oxygen therapeutics.

Original languageEnglish
Pages (from-to)322-328
Number of pages7
JournalInternational Journal of Artificial Organs
Volume24
Issue number5
DOIs
Publication statusPublished - 2001

Keywords

  • Blood substitutes
  • Hemoglobin
  • Nitric oxide
  • Polyetylene glycol
  • S-Nitorosohemoglobin
  • S-Nitrosylation

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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