Preparation and Characterization of Photoactivable Heterobifunctional Fluorescent Reagents

Koji Muramoto, Hisao Kamiya

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Two new photoactivable heterobifunctional reagents, l-azido-5-naphthalene sulfonyl chloride (ANS-C1) and the N-hydroxysuccinimide ester of l-azido-5-naphthalene sulfonyl aminopropionate (NHS-ANS-AP), were synthesized and characterized. The reagents were applicable to the group-directed modification of ligands. Upon exposure to ultraviolet light, the non-fluorescent modified ligands generated reactive nitrene intermediates which could react with neighboring molecules to form fluorescent products. This was demonstrated by the covalent modification of bovine serum albumin with ANS-AP by photolysis. NHS-ANS-AP was used for the preparation of a photore-active invertebrate lectin. Abbreviations: ANS-C1, l-azido-5-naphthalene sulfonyl chloride; NHS-ANS-AP, the N-hydroxysuccinimide ester of l-azido-5-naphthalene sulfonyl aminopropionate; BSA, bovine serum albumin; BRA, Megabalanus rosa lectin; SDS, sodium dodecyl sulfate.

    Original languageEnglish
    Pages (from-to)2695-2699
    Number of pages5
    JournalAgricultural and Biological Chemistry
    Volume48
    Issue number11
    DOIs
    Publication statusPublished - 1984 Jan

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

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