Preparation and characterization of photoactivable heterobifunctional fluorescent reagents

Koji Muramoto, Hisao Kamiya

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Two new photoactivable heterobifunctional reagents, l-azido-5-naphthalene sulfonyl chloride (ANS-Cl) and the A-hydroxysuccinimide ester of l-azido-5-naphthalene sulfonyl aminopropionate (NHS-ANS-AP), were synthesized and characterized. The reagents were applicable to the group- directed modification of ligands. Upon exposure to ultraviolet light, the non-fluorescent modified ligands generated reactive nitrene intermediates which could react with neighboring molecules to form fluorescent products. This was demonstrated by the covalent modification of bovine serum albumin with ANS-AP by photolysis. NHS-ANS-AP was used for the preparation of a photoreactive invertebrate lectin.

Original languageEnglish
Pages (from-to)2695-2699
Number of pages5
JournalAgricultural and Biological Chemistry
Volume48
Issue number11
DOIs
Publication statusPublished - 1984 Nov
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Fingerprint

Dive into the research topics of 'Preparation and characterization of photoactivable heterobifunctional fluorescent reagents'. Together they form a unique fingerprint.

Cite this