Preferential 5-Methylcytosine Oxidation in the Linker Region of Reconstituted Positioned Nucleosomes by Tet1 Protein

Seiichiro Kizaki, Tingting Zou, Yue Li, Yong Woon Han, Yuki Suzuki, Yoshie Harada, Hiroshi Sugiyama

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Tet (ten–eleven translocation) family proteins oxidize 5-methylcytosine (mC) to 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), and 5-carboxycytosine (caC), and are suggested to be involved in the active DNA demethylation pathway. In this study, we reconstituted positioned mononucleosomes using CpG-methylated 382 bp DNA containing the Widom 601 sequence and recombinant histone octamer, and subjected the nucleosome to treatment with Tet1 protein. The sites of oxidized methylcytosine were identified by bisulfite sequencing. We found that, for the oxidation reaction, Tet1 protein prefers mCs located in the linker region of the nucleosome compared with those located in the core region.

Original languageEnglish
Pages (from-to)16598-16601
Number of pages4
JournalChemistry - A European Journal
Volume22
Issue number46
DOIs
Publication statusPublished - 2016 Nov 7

Keywords

  • AFM imaging
  • DNA
  • cytosine
  • nucleosomes
  • proteins

ASJC Scopus subject areas

  • Catalysis
  • Organic Chemistry

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