Preferential 5-Methylcytosine Oxidation in the Linker Region of Reconstituted Positioned Nucleosomes by Tet1 Protein

Seiichiro Kizaki; Tingting Zou; Yue Li; Yong Woon Han; Yuki Suzuki; Yoshie Harada; Hiroshi Sugiyama

doi:10.1002/chem.201602435

Preferential 5-Methylcytosine Oxidation in the Linker Region of Reconstituted Positioned Nucleosomes by Tet1 Protein

Seiichiro Kizaki, Tingting Zou, Yue Li, Yong Woon Han, Yuki Suzuki, Yoshie Harada, Hiroshi Sugiyama

Creative Interdisciplinary Research Division

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Tet (ten–eleven translocation) family proteins oxidize 5-methylcytosine (mC) to 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), and 5-carboxycytosine (caC), and are suggested to be involved in the active DNA demethylation pathway. In this study, we reconstituted positioned mononucleosomes using CpG-methylated 382 bp DNA containing the Widom 601 sequence and recombinant histone octamer, and subjected the nucleosome to treatment with Tet1 protein. The sites of oxidized methylcytosine were identified by bisulfite sequencing. We found that, for the oxidation reaction, Tet1 protein prefers mCs located in the linker region of the nucleosome compared with those located in the core region.

Original language	English
Pages (from-to)	16598-16601
Number of pages	4
Journal	Chemistry - A European Journal
Volume	22
Issue number	46
DOIs	https://doi.org/10.1002/chem.201602435
Publication status	Published - 2016 Nov 7

Keywords

AFM imaging
DNA
cytosine
nucleosomes
proteins

ASJC Scopus subject areas

Catalysis
Organic Chemistry

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Preferential 5-Methylcytosine Oxidation in the Linker Region of Reconstituted Positioned Nucleosomes by Tet1 Protein. / Kizaki, Seiichiro; Zou, Tingting; Li, Yue; Han, Yong Woon; Suzuki, Yuki; Harada, Yoshie; Sugiyama, Hiroshi.

In: Chemistry - A European Journal, Vol. 22, No. 46, 07.11.2016, p. 16598-16601.

Research output: Contribution to journal › Article › peer-review

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keywords = "AFM imaging, DNA, cytosine, nucleosomes, proteins",

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AU - Zou, Tingting

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AU - Han, Yong Woon

AU - Suzuki, Yuki

AU - Harada, Yoshie

AU - Sugiyama, Hiroshi

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