Abstract
Tet (ten–eleven translocation) family proteins oxidize 5-methylcytosine (mC) to 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), and 5-carboxycytosine (caC), and are suggested to be involved in the active DNA demethylation pathway. In this study, we reconstituted positioned mononucleosomes using CpG-methylated 382 bp DNA containing the Widom 601 sequence and recombinant histone octamer, and subjected the nucleosome to treatment with Tet1 protein. The sites of oxidized methylcytosine were identified by bisulfite sequencing. We found that, for the oxidation reaction, Tet1 protein prefers mCs located in the linker region of the nucleosome compared with those located in the core region.
Original language | English |
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Pages (from-to) | 16598-16601 |
Number of pages | 4 |
Journal | Chemistry - A European Journal |
Volume | 22 |
Issue number | 46 |
DOIs | |
Publication status | Published - 2016 Nov 7 |
Keywords
- AFM imaging
- DNA
- cytosine
- nucleosomes
- proteins
ASJC Scopus subject areas
- Catalysis
- Organic Chemistry