TY - JOUR
T1 - Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1
AU - Nomura, Nobuo
AU - Nagase, Takahiro
AU - Miyajima, Nobuyuki
AU - Sazuka, Takashi
AU - Tanaka, Ayako
AU - Sato, Shusei
AU - Seki, Naohiko
AU - Kawarabayasi, Yutaka
AU - Ishikawa, Ken Ichi
AU - Tabata, Satoshi
PY - 1994
Y1 - 1994
N2 - By applying the protocol previously established, we isolated and sequenced full-length cDNA clones longer than 2 kb from a cDNA library of human immature myeloid cell line KG-1, and the coding sequences of 40 new genes were predicted. A computer search of the sequences indicated that 29 genes contained sequences with similarities to reported genes in the GenBank/EMBL databases. Significant transmembrane domains were identified in 9 genes, 5 of which harbored multiple hydrophobic regions. Protein motifs that matched those in the PROSITE motif database were identified in 13 genes. In terms of sequence similarities and protein motifs, 5 genes were related to transcriptional factors. Repetitive sequences were found in the 3′-untranslated region of 8 genes. Northern hybridization demonstrated that the expression of 9 genes was tissue-specific, while the remaining 31 genes were expressed ubiquitously. It was also noted that 17 genes yielded different sizes of bands possibly due to either alternative splicing or alternative initiation. The chromosomal location of these genes has been determined.
AB - By applying the protocol previously established, we isolated and sequenced full-length cDNA clones longer than 2 kb from a cDNA library of human immature myeloid cell line KG-1, and the coding sequences of 40 new genes were predicted. A computer search of the sequences indicated that 29 genes contained sequences with similarities to reported genes in the GenBank/EMBL databases. Significant transmembrane domains were identified in 9 genes, 5 of which harbored multiple hydrophobic regions. Protein motifs that matched those in the PROSITE motif database were identified in 13 genes. In terms of sequence similarities and protein motifs, 5 genes were related to transcriptional factors. Repetitive sequences were found in the 3′-untranslated region of 8 genes. Northern hybridization demonstrated that the expression of 9 genes was tissue-specific, while the remaining 31 genes were expressed ubiquitously. It was also noted that 17 genes yielded different sizes of bands possibly due to either alternative splicing or alternative initiation. The chromosomal location of these genes has been determined.
KW - Chromosomal location
KW - Full-length cDNA sequence
KW - Myeloid cell line KG-1
KW - Protein motif
KW - Unidentified human gene
KW - mRNA expression
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U2 - 10.1093/dnares/1.5.223
DO - 10.1093/dnares/1.5.223
M3 - Article
C2 - 7584044
AN - SCOPUS:12444276104
VL - 1
SP - 223
EP - 229
JO - DNA Research
JF - DNA Research
SN - 1340-2838
IS - 5
ER -