Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1

Nobuo Nomura, Takahiro Nagase, Nobuyuki Miyajima, Takashi Sazuka, Ayako Tanaka, Shusei Sato, Naohiko Seki, Yutaka Kawarabayasi, Ken Ichi Ishikawa, Satoshi Tabata

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

By applying the protocol previously established, we isolated and sequenced full-length cDNA clones longer than 2 kb from a cDNA library of human immature myeloid cell line KG-1, and the coding sequences of 40 new genes were predicted. A computer search of the sequences indicated that 29 genes contained sequences with similarities to reported genes in the GenBank/EMBL databases. Significant transmembrane domains were identified in 9 genes, 5 of which harbored multiple hydrophobic regions. Protein motifs that matched those in the PROSITE motif database were identified in 13 genes. In terms of sequence similarities and protein motifs, 5 genes were related to transcriptional factors. Repetitive sequences were found in the 3′-untranslated region of 8 genes. Northern hybridization demonstrated that the expression of 9 genes was tissue-specific, while the remaining 31 genes were expressed ubiquitously. It was also noted that 17 genes yielded different sizes of bands possibly due to either alternative splicing or alternative initiation. The chromosomal location of these genes has been determined.

Original languageEnglish
Pages (from-to)223-229
Number of pages7
JournalDNA Research
Volume1
Issue number5
DOIs
Publication statusPublished - 1994 Jan 1
Externally publishedYes

Keywords

  • Chromosomal location
  • Full-length cDNA sequence
  • Myeloid cell line KG-1
  • Protein motif
  • Unidentified human gene
  • mRNA expression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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