Prediction of lymphatic metastasis based on gene expression profile analysis after brachytherapy for early-stage oral tongue carcinoma

Hiroshi Watanabe, Kaoru Mogushi, Masahiko Miura, Ryo ichi Yoshimura, Tohru Kurabayashi, Hitoshi Shibuya, Hiroshi Tanaka, Shuhei Noda, Mayumi Iwakawa, Takashi Imai

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Background and purpose: The management of lymphatic metastasis of early-stage oral tongue carcinoma patients is crucial for its prognosis. The purpose of this study was to evaluate the predictive ability of lymphatic metastasis after brachytherapy (BRT) for early-stage tongue carcinoma based on gene expression profiling. Patients and methods: Pre-therapeutic biopsies from 39 patients with T1 or T2 tongue cancer were analyzed for gene expression signatures using Codelink Uniset Human 20K Bioarray. All patients were treated with low dose-rate BRT for their primary lesions and underwent strict follow-up under a wait-and-see policy for cervical lymphatic metastasis. Candidate genes were selected for predicting lymph-node status in the reference group by the permutation test. Predictive accuracy was further evaluated by the prediction strength (PS) scoring system using an independent validation group. Results: We selected a set of 19 genes whose expression differed significantly between classes with or without lymphatic metastasis in the reference group. The lymph-node status in the validation group was predicted by the PS scoring system with an accuracy of 76%. Conclusions: Gene expression profiling using 19 genes in primary tumor tissues may allow prediction of lymphatic metastasis after BRT for early-stage oral tongue carcinoma.

Original languageEnglish
Pages (from-to)237-242
Number of pages6
JournalRadiotherapy and Oncology
Volume87
Issue number2
DOIs
Publication statusPublished - 2008 May 1

Keywords

  • Brachytherapy
  • Gene expression profile
  • Lymphatic metastasis
  • Prediction
  • Tongue cancer

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Radiology Nuclear Medicine and imaging

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