Precise mapping and molecular characterization of the MFT1 gene involved in import of a fusion protein into mitochondria in Saccharomyces cerevisiae

Masaki Ito, Akira Yasui, Atsushi Komamine

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    Garrett et al. [Mol. Gen. Genet. 225 (1991) 483-491] recently reported that an Atp2-lacZ fusion protein was transported into mitochondria in yeast, thus identifying the MFT1 (mitochondrial fusion targeting) gene as a genomic fragment which complements a mutation (mftI) that failed in targeting a fusion protein into mitochondria [4]. They mapped this gene to the ORF, which we have independently identified as a gene homologous to the cyc07 gene, which is expressed specifically in the S phase during the plant cell cycle. We have mapped the MFT1 gene precisely and found that this gene should correspond to the neighboring ORF, rather than the ORF they identified.

    Original languageEnglish
    Pages (from-to)125-129
    Number of pages5
    JournalFEBS Letters
    Volume320
    Issue number2
    DOIs
    Publication statusPublished - 1993 Apr 5

    Keywords

    • Gene sequence
    • MFTI
    • Mitochondrial protein import
    • Saccharomyces cerevisiae
    • cyc07

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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