Precise calibration for biological acoustic impedance microscope

N. Hozumi, A. Nakano, S. Terauchi, M. Nagao, S. Yoshida, K. Kobayashi, S. Yamamoto, Y. Saijo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)


This report deals with the scanning acoustic microscope for imaging cross sectional acoustic impedance of biological soft tissues. A focused acoustic beam with a wide frequency range up to about 100 MHz was transmitted to the tissue object in contact with the "rear surface" of plastic substrate. The reflected signals from the target and reference are interpreted into local acoustic impedance. Two-dimensional profile is obtained by scanning the transducer. As the incidence is not vertical, not only longitudinal wave but also transversal wave is generated in the substrate. The error in estimated acoustic impedance assuming vertical incidence was discussed. The error is not negligible if the angle of focusing is large, or the acoustic impedance of the reference material is far different from the target. However it can be compensated, if the beam pattern and acoustic parameters of coupling medium and substrate were known. The improvement of precision brought by the compensation was ensured by using a droplet of saline solution of which acoustic impedance was known. Finally, a cerebellum tissue of rat was observed with a good precision.

Original languageEnglish
Title of host publication2007 IEEE Ultrasonics Symposium Proceedings, IUS
Number of pages4
Publication statusPublished - 2007
Event2007 IEEE Ultrasonics Symposium, IUS - New York, NY, United States
Duration: 2007 Oct 282007 Oct 31

Publication series

NameProceedings - IEEE Ultrasonics Symposium
ISSN (Print)1051-0117


Other2007 IEEE Ultrasonics Symposium, IUS
Country/TerritoryUnited States
CityNew York, NY


  • Acoustic impedanc
  • Biological tissue
  • Micro-scale imaging

ASJC Scopus subject areas

  • Acoustics and Ultrasonics


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