Practical tips for imaging ascidian embryos

Takefumi Negishi, Alex Mcdougall, Hitoyoshi Yasuo

Research output: Contribution to journalReview articlepeer-review

5 Citations (Scopus)

Abstract

Decades of studies on ascidian embryogenesis have culminated in deciphering the first gene regulatory "blueprint" for the generation of all major larval tissue types in chordates. However, the current gene regulatory network (GRN) is not well integrated with the morphogenetic and cellular processes that are also taking place during embryogenesis. Describing these processes represents a major on-going challenge, aided by recent advances in imaging and fluorescent protein (FP) technologies. In this report, we describe the application of these technologies to the developmental biology of ascidians and provide a detailed practical guide on the preparation of ascidian embryos for imaging. Development, Growth & Differentiation

Original languageEnglish
Pages (from-to)446-453
Number of pages8
JournalDevelopment Growth and Differentiation
Volume55
Issue number4
DOIs
Publication statusPublished - 2013 May

Keywords

  • Ascidian
  • Chordate
  • Embryo
  • Fluorescent protein
  • Imaging

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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