Potentiation of infectivity and pathogenesis of influenza a virus by a house dust mite protease

Takaaki Akaike, Hiroshi Maeda, Keishi Maruo, Yoshifumi Sakata, Keizo Sato

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Common house dust mites (e.g., Dermatophagoides farinae) excrete a serine-type (Df) protease. Dfprotease obtained from cultured mites enhanced viral replication in vitro via proteolytic cleavage of viral hemagglutinin (HA) into HAl and HA2, which confers potent viral infectivity. Its potency is 2- to 5-fold higher than bovine trypsin or human plasmin. Df protease also markedly accelerated virus propagation in vivo: A minute quantity of protease (estimated delivered amount, 0.8-3.2 μg) produced ∼4- to 100-fold increases in infectious virus in the mouse lung. Similar augmentation of viral replication by Df protease was observed in ferret models of nasopharyngeal infections of influenza virus. All extracts from ordinary house dust contained a serine-type protease that cleaved HA into HAl and HA2. Thus, mite protease in house dust may enhance the pathogenesis of influenza virus.

Original languageEnglish
Pages (from-to)1023-1026
Number of pages4
JournalJournal of Infectious Diseases
Volume170
Issue number4
DOIs
Publication statusPublished - 1994 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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