Potential of transfected muscle cells to contribute to DNA vaccine immunogenicity

Hidekazu Shirota, Lev Petrenko, Choongman Hong, Dennis M. Klinman

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The mechanism(s) by which DNA vaccines trigger the activation of Ag-specific T cells is incompletely understood. A series of in vivo and in vitro experiments indicates plasmid transfection stimulates muscle cells to up-regulate expression of MHC class I and costimulatory molecules and to produce multiple cytokines and chemokines. Transfected muscle cells gain the ability to directly present Ag to CD8 T cells through an IFN-regulatory factor 3-dependent process. These findings suggest that transfected muscle cells at the site of DNA vaccination may contribute to the magnitude and/or duration of the immune response initiated by professional APCs.

Original languageEnglish
Pages (from-to)329-336
Number of pages8
JournalJournal of Immunology
Volume179
Issue number1
DOIs
Publication statusPublished - 2007 Jul 1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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