TY - JOUR
T1 - Potential Activity of Carrageenan to Enhance Antibacterial Host-Defense Systems in Mice
AU - Tateda, Kazuhiro
AU - Irifune, Kenji
AU - Shimoguchi, Kazunori
AU - Tomono, Kazunori
AU - Matsumoto, Tetsuya
AU - Kaku, Mitsuo
AU - Yamaguchi, Keizo
AU - Hirakata, Yoichi
PY - 1995
Y1 - 1995
N2 - We investigated the influences of carrageenan, generally known to be a selective cytotoxic agent for macrophages, on the antibacterial host-defense systems such as the chemotactic responses to heat-killed bacteria or lipopolysaccharide in the pleural cavity and interleukin-1 production by pleural macrophages. Twenty-four hours after intraperitoneal injection of 200 mg/kg of carrageenan, there were no significant differences in the total and differential cell counts in lavaged fluid from the pleural cavity in carrageenan-pretreated and control mice. After intrapleural injection of heat-killedKlebsiella pneumoniae DT-S or lipopolysaccharide of this strain, significantly greater amounts of leukocytes, predominantly neutrophils, accumulated at 24 hrs in the pleural cavity of carrageenan-pretreated mice compared with control mice (P<0.05). Pleural macrophages obtained from carrageenan-pretreated mice produced more interleukin-1 in response to heat-killedK. pneumoniae DT-S or lipopolysaccharide than the control. Pretreatment with carrageenan significantly prolonged survival of mice against intrapleural challenge withK. pneumoniae DT-S. All 13 control mice died within 48 of inoculation with 9.2×106 organisms per mouse, whereas 11 of 13 carrageenan-pretreated mice survived (P<0.001). These results indicate that carrageenan pretreatment enhances the chemotactic responses to bacterial lipopolysaccharide in the pleural cavity and interleukin-1 production by pleural macrophages. In some experimental conditions, this polysaccharide may act as a macrophage priming agent, instead of macrophage blocker.
AB - We investigated the influences of carrageenan, generally known to be a selective cytotoxic agent for macrophages, on the antibacterial host-defense systems such as the chemotactic responses to heat-killed bacteria or lipopolysaccharide in the pleural cavity and interleukin-1 production by pleural macrophages. Twenty-four hours after intraperitoneal injection of 200 mg/kg of carrageenan, there were no significant differences in the total and differential cell counts in lavaged fluid from the pleural cavity in carrageenan-pretreated and control mice. After intrapleural injection of heat-killedKlebsiella pneumoniae DT-S or lipopolysaccharide of this strain, significantly greater amounts of leukocytes, predominantly neutrophils, accumulated at 24 hrs in the pleural cavity of carrageenan-pretreated mice compared with control mice (P<0.05). Pleural macrophages obtained from carrageenan-pretreated mice produced more interleukin-1 in response to heat-killedK. pneumoniae DT-S or lipopolysaccharide than the control. Pretreatment with carrageenan significantly prolonged survival of mice against intrapleural challenge withK. pneumoniae DT-S. All 13 control mice died within 48 of inoculation with 9.2×106 organisms per mouse, whereas 11 of 13 carrageenan-pretreated mice survived (P<0.001). These results indicate that carrageenan pretreatment enhances the chemotactic responses to bacterial lipopolysaccharide in the pleural cavity and interleukin-1 production by pleural macrophages. In some experimental conditions, this polysaccharide may act as a macrophage priming agent, instead of macrophage blocker.
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U2 - 10.1007/BF02347730
DO - 10.1007/BF02347730
M3 - Article
AN - SCOPUS:0007437748
VL - 1
SP - 59
EP - 63
JO - Journal of Infection and Chemotherapy
JF - Journal of Infection and Chemotherapy
SN - 1341-321X
IS - 1
ER -