Postmortem behaviour of rat microRNA (miRNA) as determined by comprehensive microarray analysis

Masaki Hashiyada; Kiyotaka Usui; Yoshie Hayashizaki; Jun Sakai; Masato Funayama

doi:10.1016/j.fsigss.2011.08.105

Postmortem behaviour of rat microRNA (miRNA) as determined by comprehensive microarray analysis

Masaki Hashiyada, Kiyotaka Usui, Yoshie Hayashizaki, Jun Sakai, Masato Funayama

MED - Public Health

Research output: Contribution to journal › Article › peer-review

Abstract

We determined a number of miRNAs exist in rats after death using comprehensive microarray analysis with 350 rat miRNAs probes. Our analysis revealed that 237 miRNAs (67.7%) were under the threshold level and 105 (30%) were expressed until 48. h after death. Some miRNAs showed the unstable levels of expression at postmortem. Further investigations using quantitative PCR analysis and animal experiments are required before forensic application of this knowledge, such as estimation of time of death or quantification of stress.

Original language	English
Pages (from-to)	e210-e211
Journal	Forensic Science International: Genetics Supplement Series
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/j.fsigss.2011.08.105
Publication status	Published - 2011 Dec

Keywords

MicroRNA (miRNA)
Microarray
Postmortem
Rat

ASJC Scopus subject areas

Pathology and Forensic Medicine
Genetics

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abstract = "We determined a number of miRNAs exist in rats after death using comprehensive microarray analysis with 350 rat miRNAs probes. Our analysis revealed that 237 miRNAs (67.7%) were under the threshold level and 105 (30%) were expressed until 48. h after death. Some miRNAs showed the unstable levels of expression at postmortem. Further investigations using quantitative PCR analysis and animal experiments are required before forensic application of this knowledge, such as estimation of time of death or quantification of stress.",

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AU - Hayashizaki, Yoshie

AU - Sakai, Jun

AU - Funayama, Masato

PY - 2011/12

Y1 - 2011/12

N2 - We determined a number of miRNAs exist in rats after death using comprehensive microarray analysis with 350 rat miRNAs probes. Our analysis revealed that 237 miRNAs (67.7%) were under the threshold level and 105 (30%) were expressed until 48. h after death. Some miRNAs showed the unstable levels of expression at postmortem. Further investigations using quantitative PCR analysis and animal experiments are required before forensic application of this knowledge, such as estimation of time of death or quantification of stress.

AB - We determined a number of miRNAs exist in rats after death using comprehensive microarray analysis with 350 rat miRNAs probes. Our analysis revealed that 237 miRNAs (67.7%) were under the threshold level and 105 (30%) were expressed until 48. h after death. Some miRNAs showed the unstable levels of expression at postmortem. Further investigations using quantitative PCR analysis and animal experiments are required before forensic application of this knowledge, such as estimation of time of death or quantification of stress.

KW - MicroRNA (miRNA)

KW - Microarray

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KW - Rat

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Postmortem behaviour of rat microRNA (miRNA) as determined by comprehensive microarray analysis

Abstract

Keywords

ASJC Scopus subject areas

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