Post-transcriptional regulation of breast cancer resistance protein after intestinal ischemia-reperfusion

Jiro Ogura, Masaki Kobayashi, Shirou Itagaki, Takeshi Hirano, Ken Iseki

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Breast cancer resistance protein (BCRP), the product of the ABCG2 gene, is a recently identified ATP binding cassette half-transporter. BCRP is expressed in a variety of tumor cells and many normal human tissues. In the small intestine, BCRP can limit the influx and facilitate the efflux to prevent intracellular accumulation of BCRP substrates. Ischemia-reperfusion (I/R) induces the release of reactive oxygen species, and organs are severely damaged by I/R. It has been shown that the expression of transporters was altered in the organ after I/R. The present study was undertaken to clarify the expression of BCRP after intestinal I/R. We showed that the expression level of Bcrp was significantly decreased at 1 h after I/R. Bcrp mRNA level was not altered at 1 h after I/R. These results suggest that Bcrp expression was regulated by a post-transcriptional regulation mechanism after intestinal I/R. Bcrp mRNA level was increased at 24 h after I/R, and the expression level of Bcrp protein was of the same level or slightly increased compared with sham operated-rats. Bcrp was slightly located at the intestinal membrane at 24 h after intestinal I/R. These results suggested that Bcrp was not translocated to the intestinal membrane after intestinal I/R. There is little information on post-transcriptional regulation compared with information on transcriptional regulation. In this study, it was shown that Bcrp expression is regulated by post-transcriptional regulation after intestinal I/R. These results of this study may provide important information for further studies aimed at revealing the biological function of Bcrp.

Original languageEnglish
Pages (from-to)1032-1035
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume31
Issue number5
DOIs
Publication statusPublished - 2008 May

Keywords

  • Breast cancer resistance protein
  • Intestinal ischemia-reperfusion
  • Regulation

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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