Post-transcriptional control of the cyanobacterial hspA heat-shock induction

Kouji Kojima, Hitoshi Nakamoto

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The hspA gene encodes a small heat-shock protein in the thermophilic cyanobacterium Synechococcus vulcanus. To gain insight into the post-transcriptional regulation, hspA was expressed in Escherichia coli. HspA was induced upon a temperature upshift from 30 to 42°C, although the hspA transcription in E. coli occurred constitutively at both 30 and 42°C. Neither replacement of the native hspA promoter with the lacZ promoter nor the addition of rifampin abolished the heat induction. Thus, the primary form of regulation of the heat induction is at the post-transcriptional level. Analyses of expression of a series of the transcriptional and translational hspA-lacZ fusions confirmed the constitutive transcription, and demonstrated that the heat induction occurred only in the translational fusions. They further indicated the presence of regulatory elements involved in the translational regulation. An element in the 5′-untranslated region of the hspA mRNA suppressed the translation, while that in the hspA coding region was required for the de-repression of the translation and for thermal regulation.

Original languageEnglish
Pages (from-to)583-588
Number of pages6
JournalBiochemical and biophysical research communications
Volume331
Issue number2
DOIs
Publication statusPublished - 2005 Jun 3

Keywords

  • 16S ribosomal RNA
  • Cyanobacterium
  • Heat-shock protein
  • HspA
  • Post-transcriptional regulation
  • Small heat-shock protein
  • Synechococcus vulcanus

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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