Post-illumination transient O2-uptake is driven by photorespiration in tobacco leaves

Takehiro Sejima, Hitomi Hanawa, Ginga Shimakawa, Daisuke Takagi, Yuji Suzuki, Hiroshi Fukayama, Amane Makino, Chikahiro Miyake

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This study aims to elucidate the molecular mechanism for the transient increase in the O2-uptake rate in tobacco (Nicotiana tabacum cv Xanthi) leaves after turning off actinic lights (ALs). The photosynthetic O2 evolution rate reaches a maximum shortly after the onset of illumination with ALs and then decreases to zero in atmospheric CO2/O2 conditions. After turning off the ALs, tobacco leaves show a transient increase in the O2-uptake rate, the post-illumination transient O2-uptake, and thereafter, the O2-uptake rate decreases to the level of the dark-respiration rate. Photosynthetic linear electron flow, evaluated as the quantum yield of photosystem II [Y(II)], maintained a steady-state value distinct from the photosynthetic O2-evolution rate. In high-[CO2] conditions, the photosynthetic O2-evolution rate and Y(II) showed a parallel behavior, and the post-illumination transient O2-uptake was suppressed. On the other hand, in maize leaves (a C4 plant), even in atmospheric CO2/O2 conditions, Y(II) paralleled the photosynthetic O2-evolution rate and the post-illumination transient O2-uptake was suppressed. Hypothesizing that the post-illumination transient O2-uptake is driven by C3 plant photorespiration in tobacco leaves, we calculated both the ribulose 1,5-bisphosphate carboxylase- and oxygenase-rates (Vc and Vo) from photosynthetic O2-evolution and the post-illumination transient O2-uptake rates. These values corresponded to those estimated from simultaneous chlorophyll fluorescence/O2-exchange analysis. Furthermore, the H+-consumption rate for ATP synthesis in both photosynthesis and photorespiration, calculated from both Vc and Vo that were estimated from chlorophyll fluorescence/CO2-exchange analysis, showed a positive linear relationship with the dissipation rate of the electrochromic shift signal. Thus, these findings support our hypothesis.

Original languageEnglish
Pages (from-to)227-238
Number of pages12
JournalPhysiologia Plantarum
Volume156
Issue number2
DOIs
Publication statusPublished - 2016 Feb 1

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

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    Sejima, T., Hanawa, H., Shimakawa, G., Takagi, D., Suzuki, Y., Fukayama, H., Makino, A., & Miyake, C. (2016). Post-illumination transient O2-uptake is driven by photorespiration in tobacco leaves. Physiologia Plantarum, 156(2), 227-238. https://doi.org/10.1111/ppl.12388