TY - JOUR
T1 - Post-genomic approach based discovery of alkylresorcinols from a cricket-associated fungus, Penicillium soppi
AU - Kaneko, Akiho
AU - Morishita, Yohei
AU - Tsukada, Kento
AU - Taniguchi, Tohru
AU - Asai, Teigo
N1 - Funding Information:
We thank Prof. K. Gomi (Tohoku University) and Prof. K. Kitamoto (The University of Tokyo) for providing the expression vectors and the fungal strain. This work was supported by JSPS KAKENHI (grant no. 17H05055 and 17H05428 to T. Asai from the Japan Society for the Promotion of Science (JSPS)), the Naito Foundation and Astellas Foundation for Research on Metabolic Disorders.
Publisher Copyright:
© 2019 The Royal Society of Chemistry.
PY - 2019
Y1 - 2019
N2 - Polyketide synthase (PKS) gene-guided genome mining in a cricket-associated fungus, Penicillium soppi, revealed a cryptic biosynthetic gene cluster that contained a highly reducing PKS (HR-PKS), a type III PKS, and a P450 gene. Heterologous expression of the cluster in Aspergillus oryzae led to the isolation of novel alkylresorcinols with a unique Z,E,Z-triene motif. This study displays an unusual biosynthetic mechanism of an HR-PKS and a new releasing mechanism via a type III PKS in fungi.
AB - Polyketide synthase (PKS) gene-guided genome mining in a cricket-associated fungus, Penicillium soppi, revealed a cryptic biosynthetic gene cluster that contained a highly reducing PKS (HR-PKS), a type III PKS, and a P450 gene. Heterologous expression of the cluster in Aspergillus oryzae led to the isolation of novel alkylresorcinols with a unique Z,E,Z-triene motif. This study displays an unusual biosynthetic mechanism of an HR-PKS and a new releasing mechanism via a type III PKS in fungi.
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U2 - 10.1039/c9ob00807a
DO - 10.1039/c9ob00807a
M3 - Article
C2 - 31086874
AN - SCOPUS:85066433889
SN - 1477-0520
VL - 17
SP - 5239
EP - 5243
JO - Organic and Biomolecular Chemistry
JF - Organic and Biomolecular Chemistry
IS - 21
ER -