Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5

Takashi Ashino, Haruyo Ohkubo-Morita, Masayuki Yamamoto, Takemi Yoshida, Satoshi Numazawa

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Cytochrome P450 gene expression is altered by various chemical compounds. In this study, we used nuclear factor erythroid 2-related factor 2 (Nrf2)-deficient (Nrf2-/-) mice to investigate the involvement of Nrf2 in Cyp2b10 and Cyp2a5 gene expression. Phorone, an Nrf2 activator, strongly increased Cyp2b10 and Cyp2a5 mRNA as well as Nrf2 target genes, including NAD(P)H-quinone oxidoreductase-1 and heme oxygenase-1, in wild-type mouse livers 8h after treatment. The phorone-induced mRNA levels in Nrf2-/- mouse livers were lower than that in wild-type mouse livers. Nrf2-/- mice showed attenuated Cyp2b10 and Cyp2a5 induction by phenobarbital, a classical Cyp2b inducer. These findings suggest that the Nrf2 pathway is involved in Cyp2b10 and Cyp2a5 gene expression.

Original languageEnglish
Pages (from-to)284-288
Number of pages5
JournalRedox Biology
Volume2
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • Cyp2a5
  • Cyp2b10
  • Mouse
  • Nuclear-factor erythroid 2-related factor 2
  • Phenobarbital
  • Phorone

ASJC Scopus subject areas

  • Organic Chemistry
  • Clinical Biochemistry

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