Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5

Takashi Ashino; Haruyo Ohkubo-Morita; Masayuki Yamamoto; Takemi Yoshida; Satoshi Numazawa

doi:10.1016/j.redox.2013.12.025

Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5

Takashi Ashino, Haruyo Ohkubo-Morita, Masayuki Yamamoto, Takemi Yoshida, Satoshi Numazawa

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Cytochrome P450 gene expression is altered by various chemical compounds. In this study, we used nuclear factor erythroid 2-related factor 2 (Nrf2)-deficient (Nrf2^-/-) mice to investigate the involvement of Nrf2 in Cyp2b10 and Cyp2a5 gene expression. Phorone, an Nrf2 activator, strongly increased Cyp2b10 and Cyp2a5 mRNA as well as Nrf2 target genes, including NAD(P)H-quinone oxidoreductase-1 and heme oxygenase-1, in wild-type mouse livers 8h after treatment. The phorone-induced mRNA levels in Nrf2^-/- mouse livers were lower than that in wild-type mouse livers. Nrf2^-/- mice showed attenuated Cyp2b10 and Cyp2a5 induction by phenobarbital, a classical Cyp2b inducer. These findings suggest that the Nrf2 pathway is involved in Cyp2b10 and Cyp2a5 gene expression.

Original language	English
Pages (from-to)	284-288
Number of pages	5
Journal	Redox Biology
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.redox.2013.12.025
Publication status	Published - 2014

Keywords

Cyp2a5
Cyp2b10
Mouse
Nuclear-factor erythroid 2-related factor 2
Phenobarbital
Phorone

ASJC Scopus subject areas

Organic Chemistry
Clinical Biochemistry

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10.1016/j.redox.2013.12.025

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title = "Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5",

abstract = "Cytochrome P450 gene expression is altered by various chemical compounds. In this study, we used nuclear factor erythroid 2-related factor 2 (Nrf2)-deficient (Nrf2-/-) mice to investigate the involvement of Nrf2 in Cyp2b10 and Cyp2a5 gene expression. Phorone, an Nrf2 activator, strongly increased Cyp2b10 and Cyp2a5 mRNA as well as Nrf2 target genes, including NAD(P)H-quinone oxidoreductase-1 and heme oxygenase-1, in wild-type mouse livers 8h after treatment. The phorone-induced mRNA levels in Nrf2-/- mouse livers were lower than that in wild-type mouse livers. Nrf2-/- mice showed attenuated Cyp2b10 and Cyp2a5 induction by phenobarbital, a classical Cyp2b inducer. These findings suggest that the Nrf2 pathway is involved in Cyp2b10 and Cyp2a5 gene expression.",

keywords = "Cyp2a5, Cyp2b10, Mouse, Nuclear-factor erythroid 2-related factor 2, Phenobarbital, Phorone",

author = "Takashi Ashino and Haruyo Ohkubo-Morita and Masayuki Yamamoto and Takemi Yoshida and Satoshi Numazawa",

year = "2014",

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language = "English",

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AU - Ashino, Takashi

AU - Ohkubo-Morita, Haruyo

AU - Yamamoto, Masayuki

AU - Yoshida, Takemi

AU - Numazawa, Satoshi

PY - 2014

Y1 - 2014

N2 - Cytochrome P450 gene expression is altered by various chemical compounds. In this study, we used nuclear factor erythroid 2-related factor 2 (Nrf2)-deficient (Nrf2-/-) mice to investigate the involvement of Nrf2 in Cyp2b10 and Cyp2a5 gene expression. Phorone, an Nrf2 activator, strongly increased Cyp2b10 and Cyp2a5 mRNA as well as Nrf2 target genes, including NAD(P)H-quinone oxidoreductase-1 and heme oxygenase-1, in wild-type mouse livers 8h after treatment. The phorone-induced mRNA levels in Nrf2-/- mouse livers were lower than that in wild-type mouse livers. Nrf2-/- mice showed attenuated Cyp2b10 and Cyp2a5 induction by phenobarbital, a classical Cyp2b inducer. These findings suggest that the Nrf2 pathway is involved in Cyp2b10 and Cyp2a5 gene expression.

AB - Cytochrome P450 gene expression is altered by various chemical compounds. In this study, we used nuclear factor erythroid 2-related factor 2 (Nrf2)-deficient (Nrf2-/-) mice to investigate the involvement of Nrf2 in Cyp2b10 and Cyp2a5 gene expression. Phorone, an Nrf2 activator, strongly increased Cyp2b10 and Cyp2a5 mRNA as well as Nrf2 target genes, including NAD(P)H-quinone oxidoreductase-1 and heme oxygenase-1, in wild-type mouse livers 8h after treatment. The phorone-induced mRNA levels in Nrf2-/- mouse livers were lower than that in wild-type mouse livers. Nrf2-/- mice showed attenuated Cyp2b10 and Cyp2a5 induction by phenobarbital, a classical Cyp2b inducer. These findings suggest that the Nrf2 pathway is involved in Cyp2b10 and Cyp2a5 gene expression.

KW - Cyp2a5

KW - Cyp2b10

KW - Mouse

KW - Nuclear-factor erythroid 2-related factor 2

KW - Phenobarbital

KW - Phorone

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Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5

Abstract

Keywords

ASJC Scopus subject areas

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