Possible immune functions of congerin, a mucosal galectin, in the intestinal lumen of Japanese conger eel

O. Nakamura, Y. Inaga, S. Suzuki, S. Tsutsui, Koji Muramoto, H. Kamiya, T. Watanabe

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    Congerin, a mucosal galectin of the Japanese conger eel, provides chemical fortification through its agglutinating and opsonizing activity. Congerin is produced in the epidermis, and the epithelia of the oral cavity to the esophagus, but not in the stomach or intestine. We hypothesized that congerin secreted from the upper digestive tract can reach and function in the intestinal lumen. We found that congerin possessed marked resistance against digestion by gastric and enteric enzymes of conger eel. It was not degraded until 6 h of incubation with stomach extract or intestinal digestion juice. Western blotting demonstrated that congerin essentially remained in the intestinal mucus. The mucus agglutinated rabbit erythrocytes, and the agglutination was hampered by anti-congerin antibody. Furthermore, congerin could bind to some enteric bacteria. These results support the above hypothesis.

    Original languageEnglish
    Pages (from-to)683-692
    Number of pages10
    JournalFish and Shellfish Immunology
    Volume23
    Issue number3
    DOIs
    Publication statusPublished - 2007 Sep 1

    Keywords

    • Congerin
    • Galectin
    • Intestine
    • Japanese conger eel
    • Mucosal immunity

    ASJC Scopus subject areas

    • Environmental Chemistry
    • Aquatic Science

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