Possibility of pre-adjusting exogenous liquid fibrin glue prior to the surgical operation

Background: Liquid type fibrin glue should, in principle, be prepared immediately before use. We studied the feasiblity of dissolving fibrin glue in advance of surgery. Materials and Methods: Solutions A and B were prepared using Beriplast (Aventis Pharma Co. Ltd., Tokyo, Japan). The viscosity of solitions A and B was measured throughout the time course; tensile strength was measured using the swine skin section method, and the potency of fibrinogen, thrombin, and factor XIII after preparation were also examined; and bacteria reproduction in both solutions was studied under the following 3 conditions: at room temperature (20 to 25°C), preserved in a cold place (6°C), and at 37°C. Results: (1) The tensile strength was maintained for 10 days under all of the above conditions, without reduction. (2) The viscosity of solution A did not change markedly immediately after dissolution to 3 days after dissolution at any temperature. The viscosity of solution B did not change immediately after dissolution and even 10 days after dissolution. (3) Time-course changes in fibrinogen and factor XIII activity were mild, whereas thrombin activity decreased to 48.9 ∼ 63% at 3 days in all samples, and activity continued to decrease gradually. (4) Tests conducted immediately and 10 days after preparation demonstrated the sterility of both solutions. Conclusion: It is feasible to prepare fibrin glue in advance of use, given its effectiveness as a biological adhesive.