Positive expressions of N-acetylglucosaminyltransferase-V (GnT-V) and β1-6 branching N-linked oligosaccharides in human testicular germ cells diminish during malignant transformation and progression

Atsushi Kyan, Noritaka Kamimura, Shigeru Hagisawa, Shingo Hatakeyama, Takuya Koie, Takahiro Yoneyama, Yoichi Arai, Hiroaki Nakagawa, Shin Ichiro Nishimura, Eiji Miyoshi, Yasuhiro Hashimoto, Chikara Ohyama

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

N-acetylglucosaminyltransferase-V (GnT-V) is an enzyme that catalyzes β1-6 branching of N-acetylglucosamine on asparagines (N)-linked oligosaccharides of cell proteins. We examined the implication of GnT-V and β1-6 branching N-linked oligosaccharide expression in human testicular germ cells during malignant transformation and cancer progression. We analyzed immuhistochemically orchiectomy specimens of 130 patients with testicular germ cell tumors (TGCT) using anti-GnT-V monoclonal antibody, and compared GnT-V expression with clinicopathological features. N-linked oligosaccharide structural analysis was also performed to confirm the oligosaccharide profile produced by GnT-V. GnT-V was positive in all normal testis samples. This positive incidence declined in TGCT according to clinical stage; 16/71 (22.5%) in stage I, and 3/59 (5.1%) in stage II/III (p=0.015, x2 test). When divided into pathological subtypes, GnT-V positive incidences in stage I seminoma, stage II/III seminoma, stage I non-seminomatous germ cell tumor (NSGCT), and stage II/III NSGCT were 3/43 (7%), 0/22 (0%), 13/28 (46.4%), and 3/37 (8.1%), respectively. In stage I NSGCT, patients with GnT-V-negative rumor samples were at a significantly higher risk of recurrence than those with GnT-V-positive tumors (p=0.015, log-rank test). N-linked oligosaccharide structural analysis revealed that a normal testis has three kinds of β-6 branching N-linked oligosaccharides, all of which are downregulated in TGCT tissues. These results suggest that GnT-V and β1-6 branching N-linked oligosaccharide expressions are downregulated during carcinogenesis and progression of human TGCT. GnT-V may be a promising recurrence predictor for stage I NSGCT.

Original languageEnglish
Pages (from-to)129-134
Number of pages6
JournalInternational journal of oncology
Volume32
Issue number1
DOIs
Publication statusPublished - 2008 Jan
Externally publishedYes

Keywords

  • GnT-V
  • N-glycan
  • Testicular germ cell tumor
  • Testis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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