Positive and negative regulation of the human heme oxygenase-1 gene expression in cultured cells

Shigeru Takahashi, Yuji Takahashi, Kazumi Ito, Tadahiro Nagano, Shigeki Shibahara, Takashi Miura

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

To elucidate the regulation of the human heme oxygenase-1 (hHO-1) gene expression, we assessed approximately 4 kb of the 5'-flanking region of the hHO-1 gene for basal promoter activity and sequenced approximately 2 kb of the 5'-flanking region. A series of deletion mutants of the 5'-flanking region linked to the luciferase gene was constructed. Basal level expression of these constructs was tested in HepG2 human hepatoma cells and HeLa cervical cancer cells. By measuring luciferase activity, which was transiently expressed in the transfected cells, we found a positive regulatory region at position -1976 to -1655 bp. This region functions in HepG2 cells but not in HeLa cells. A negative regulatory region was also found at position -981 to -412 bp that functions in both HepG2 cells and HeLa cells. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)231-235
Number of pages5
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1447
Issue number2-3
DOIs
Publication statusPublished - 1999 Oct 28
Externally publishedYes

Keywords

  • Gene regulation
  • Heme oxygenase
  • Liver

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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